JCRB0541 TIG-111

Cell information

Important Notice(s)

Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0541 Cell Name   TIG-111
Profile   Normal diploid fibroblast from human skin with limited lifespan. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   34
Identity   available Tissue for Primary Cancer   skin
Case history   normal Metastasis   
Tissue Metastasized    Genetics   normal diploid fibroblast (2n-46, XX)
Life Span   finite(PD38) Crisis PDL   PD38
Morphology   fibroblast Character   normal diploid
Classify   normal Established by   TMIG
Registered by   TMIG Regulation for Distribution   
Comment    Year   2004
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   NT
Cell Number on Passage   split ratio = 1/4 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   *

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3196
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0541 Cell Name   TIG-111
LOT No.   08152014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   2.5 - 3.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   D.T. = approx. 31 hrs Cell Number in Vial (cells/1ml)   7.7 x 10^5
Viability at cell freezing (%)   93.8 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   22.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   93.8 Additional information   
Cell No.   JCRB0541 Cell Name   TIG-111
LOT No.   08082024 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   6.2 - 7.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 52 hrs. Cell Number in Vial (cells/1ml)   9.4 x 10^5
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   19.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:11,13
D7S820:11
D16S539:10,11
VWA:18,19
TH01:9,9.3
AM:X
TPOX:8,9
CSF1PO:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
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