-Detailed Information [JCRB0234.0]-

JCRB0234.0 TYK-nu

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0234.0	Cell Name	TYK-nu
Profile	undifferentiated carcinoma	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age
Identity	available	Tissue for Primary Cancer	ovary
Case history		Metastasis
Tissue Metastasized		Genetics	ovary, undifferentiated carcinoma
Life Span		Crisis PDL
Morphology		Character	ovary, undifferentiated carcinoma
Classify		Established by	Yoshiya,N.
Registered by	Egashira, Y.	Regulation for Distribution	Make notice to the originator on distribution.
Comment		Year	1987
Medium	Eagle's minimal essential medium with 10% fetal calf serum	Methods for Passages	Cells are treated with 0.25 % trypsin, 0.1-0.01 % EDTA (4/1)
Cell Number on Passage	2x10^4 cells/ml	Race
CO2 Conc.	5 %	Tissue Sampling	ovary
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1776
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	909774

Reference
Pubmed id:3782955	[Establishment of a cell line from human ovarian cancer (undifferentiated carcinoma of FIGO classification) and analysis of its cell-biological characteristics and sensitivity to anticancer drugs]. Yoshiya N Nihon Sanka Fujinka Gakkai Zasshi. 1986 Oct;38(10):1747-53

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

012099
100794
07182013
08192016
02252019
02132023
01202025

Cell No.	JCRB0234.0	Cell Name
LOT No.	012099	Lot Specification	distribution
Medium	Eagle's minimal essential medium + 10% fetal calf serum (Biowhittaker 6M0121)	Temperature	37 C
Cell Density at Seeding	5 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	ca 50hrs	Cell Number in Vial (cells/1ml)	1.8 X10^6
Viability at cell freezing (%)	86%	Antibiotics Used	free
Passage Number	P9	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0234.0	Cell Name
LOT No.	100794	Lot Specification	distribution
Medium	MEM + 10 % FCS	Temperature	37
Cell Density at Seeding	1/5 - 1/20	Methods for Passages	0.25% trypsin, 0.02% EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	1.1 x 10^6
Viability at cell freezing (%)	96.0	Antibiotics Used	free
Passage Number	P106	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	AST, G6PD, LD, MD, MPI, NP, PEPB
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0234.0	Cell Name	TYK-nu
LOT No.	07182013	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 10% fetal calf serum (FBS; GIBCO Cat. # 10099)	Temperature	37C
Cell Density at Seeding	6.4 x 10^4 cells/mL	Methods for Passages	Cells are treated with 0.25 % trypsin, 0.02% EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	2.2 x 10^6 (cells/1
Viability at cell freezing (%)		Antibiotics Used	free
Passage Number	P12	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.	5%
Viability immediately after thawing (%)	92.4	Additional information

Cell No.	JCRB0234.0	Cell Name	TYK-nu
LOT No.	08192016	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	1.0 - 1.5 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 44 hrs.	Cell Number in Vial (cells/1ml)	1.7 x 10^6
Viability at cell freezing (%)	83	Antibiotics Used	free
Passage Number	P12	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	83	Additional information

Cell No.	JCRB0234.0	Cell Name	TYK-nu
LOT No.	02252019	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	0.5 - 1.5 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 26 hrs.	Cell Number in Vial (cells/1ml)	3.4 x 10^6
Viability at cell freezing (%)	95	Antibiotics Used	free
Passage Number	P12	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	90	Additional information

Cell No.	JCRB0234.0	Cell Name	TYK-nu
LOT No.	02132023	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437)	Temperature	37 C
Cell Density at Seeding	0.4-1.2 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	28 - 34 hrs.	Cell Number in Vial (cells/1ml)	2.3 x 10^6
Viability at cell freezing (%)	98.1	Antibiotics Used	free
Passage Number	P12	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12,13 D13S317:10,11 D7S820:10 D16S539:9,10 VWA:14,16 TH01:9 AM:X TPOX:9,11 CSF1PO:12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	5% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	95.1	Additional information

Cell No.	JCRB0234.0	Cell Name	TYK-nu
LOT No.	01202025	Lot Specification	distribution
Medium	Eagle's minimum essential medium (GIBCO: Cat.　No.11095-080) with 10% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K)	Temperature	37 C
Cell Density at Seeding	1.8-1.9x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.1x10^6
Viability at cell freezing (%)	100	Antibiotics Used	free
Passage Number	p12	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12,13 D13S317:10,11 D7S820:10 D16S539:9,10 VWA:14,16 TH01:9 AM:X TPOX:9,11 CSF1PO:12
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	97	Additional information

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