JCRB1078 TMH-1
Cell information
Important Notice(s)Cross contamination in JCRB1078 TMH-1 cells.
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1078 | Cell Name | TMH-1 |
|---|---|---|---|
| Profile | Human adherent monolayer cell line with multidrug resistance obtained from a benign goiter and chronic thyroiditis. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 79 |
| Identity | available | Tissue for Primary Cancer | thyroid |
| Case history | benign goiter and chronic thyroiditis, radiotherapy at a total dose of 43.5 Gy without chemotherapy | Metastasis | |
| Tissue Metastasized | Genetics | Choromosome No. ditributes from 40 to 120. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | spindle-shaped with giant cells | Character | Doubling time is 74 hours. Multidrug resistance. |
| Classify | tumor | Established by | Sekiguchi,M. et.al. |
| Registered by | Sekiguchi,M. | Regulation for Distribution | not applied |
| Comment | Cross contamination of IHH-4 cell line is suspected. | Year | 2003 |
| Medium | 1 to 1 mixture of Dulbecco's modified Eagle's medium and RPMI1640 medium containing 10% heat inactivated fetal bovine serum. | Methods for Passages | Cells are harvested after treatment with 0.25% trypsin. |
| Cell Number on Passage | 10^4 cells/ml | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | thyroid(goiter) |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:11686581 | Biological characteristics and chemosensitivity profile of four human anaplastic thyroid carcinoma cell lines. Sekiguchi M,Shiroko Y,Arai T,Kishino T,Sugawara I,Kusakabe T,Suzuki T,Yamashita T,Obara T,Ito K,Hasumi K Biomed Pharmacother. 2001 Oct;55(8):466-74 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1078 | Cell Name | TMH-1 |
|---|---|---|---|
| LOT No. | 11272003 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum (Intergen RB52305). | Temperature | 37 C |
| Cell Density at Seeding | 1.0 x 10^4 cells/sq.cm. | Methods for Passages | Cells are harvested after treatment with 0.25% trypsin. Subculture once a week. Medium change once a week. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.3 x 10^6 |
| Viability at cell freezing (%) | 99.0 | Antibiotics Used | free |
| Passage Number | P304 | PDL | 42 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:9,13 D13S317:10,13 D7S820:9,10 D16S539:9,11 VWA:16,18 TH01:9 AM:X TPOX:11 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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