JCRB0232 SKG-IIIa
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0232 | Cell Name | SKG-IIIa |
|---|---|---|---|
| Profile | cervical epidermoid carcinoma, clear cell type | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | uterine cervix |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | cervix | |
| Life Span | infinite | Crisis PDL | |
| Morphology | Character | Growth rate is slow. | |
| Classify | Established by | Nozawa,S. | |
| Registered by | Egashira, T. | Regulation for Distribution | |
| Comment | Tumour markers, TA-4, CA-4, TPA, placental ALP, produed. | Year | 1987 |
| Medium | Ham's F12 medium with 10% fetal calf serum. | Methods for Passages | Cells are treated with 0.1 % trypsin and 0.02 % EDTA. |
| Cell Number on Passage | 2x10^4 cells/ml | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | cervix |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | Integration of HPV16 to host genome (~one copy/cell) was reported (Int J Cancer 1986;37:499) | ||||||||
| Reference | |
|---|---|
| Pubmed id:6699456 | [Biological properties of two newly established cell lines (SKG-3a,3b) from a human uterine cervical epidermoid carcinoma]. Udagawa Y,Nozawa S,Tsukazaki K,Ohta H,Akiba T,Kurihara S Nihon Sanka Fujinka Gakkai Zasshi. 1984 Feb;36(2):237-46 |
| Pubmed id:6604031 | Sodium butyrate produces concordant expression of "early placental" alkaline phosphatase, pregnancy-specific beta 1-glycoprotein and human chronic gonadotropin beta-subunit in a newly established uterine cervical cancer cell line (SKG-IIIa). Nozawa S,Engvall E,Kano S,Kurihara S,Fishman WH Int J Cancer. 1983 Sep 15;32(3):267-72 |
| Pubmed id:6600963 | Newly established uterine cervical cancer cell line (SKG-III) with Regan isoenzyme, human chorionic gonadotropin beta-subunit, and pregnancy-specific beta 1-glycoprotein phenotypes. Nozawa S,Udagawa Y,Ohta H,Kurihara S,Fishman WH Cancer Res. 1983 Apr;43(4):1748-60 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0232 | Cell Name | SKG-IIIa |
|---|---|---|---|
| LOT No. | 11042022 | Lot Specification | distribution |
| Medium | Ham's F-12 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322) | Temperature | 37 C |
| Cell Density at Seeding | approx. 2.5 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | 41 - 83 hrs. | Cell Number in Vial (cells/1ml) | 2.2 x 10^6 |
| Viability at cell freezing (%) | 97.9 | Antibiotics Used | free |
| Passage Number | P23 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:10,11 D13S317:12 D7S820:10,12 D16S539:9,11 VWA:16 TH01:6,9 AM:X TPOX:8,11 CSF1PO:11,12 |
|
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 5% DMSO, 20% FBS - Ham's F-12 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 97.3 | Additional information |