JCRB0232 SKG-IIIa

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0232 Cell Name   SKG-IIIa
Profile   cervical epidermoid carcinoma, clear cell type Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   uterine cervix
Case history    Metastasis   
Tissue Metastasized    Genetics   cervix
Life Span   infinite Crisis PDL   
Morphology    Character   Growth rate is slow.
Classify    Established by   Nozawa,S.
Registered by   Egashira, T. Regulation for Distribution   
Comment   Tumour markers, TA-4, CA-4, TPA, placental ALP, produed. Year   1987
Medium   Ham's F12 medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.1 % trypsin and 0.02 % EDTA.
Cell Number on Passage   2x10^4 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   cervix
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes Integration of HPV16 to host genome (~one copy/cell) was reported (Int J Cancer 1986;37:499)

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1704
Reference
Pubmed id:6699456[Biological properties of two newly established cell lines (SKG-3a,3b) from a human uterine cervical epidermoid carcinoma].
Udagawa Y,Nozawa S,Tsukazaki K,Ohta H,Akiba T,Kurihara S
Nihon Sanka Fujinka Gakkai Zasshi. 1984 Feb;36(2):237-46
Pubmed id:6604031Sodium butyrate produces concordant expression of "early placental" alkaline phosphatase, pregnancy-specific beta 1-glycoprotein and human chronic gonadotropin beta-subunit in a newly established uterine cervical cancer cell line (SKG-IIIa).
Nozawa S,Engvall E,Kano S,Kurihara S,Fishman WH
Int J Cancer. 1983 Sep 15;32(3):267-72
Pubmed id:6600963Newly established uterine cervical cancer cell line (SKG-III) with Regan isoenzyme, human chorionic gonadotropin beta-subunit, and pregnancy-specific beta 1-glycoprotein phenotypes.
Nozawa S,Udagawa Y,Ohta H,Kurihara S,Fishman WH
Cancer Res. 1983 Apr;43(4):1748-60

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0232 Cell Name   SKG-IIIa
LOT No.   11042022 Lot Specification   distribution
Medium   Ham's F-12 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322) Temperature   37 C
Cell Density at Seeding   approx. 2.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   41 - 83 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   97.9 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,11
D13S317:12
D7S820:10,12
D16S539:9,11
VWA:16
TH01:6,9
AM:X
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - Ham's F-12 CO2 Conc.   5%
Viability immediately after thawing (%)   97.3 Additional information   
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