-Detailed Information [JCRB0226]-

JCRB0226 COLO201

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0226	Cell Name	COLO201
Profile	adenocarcinoma	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age
Identity	available	Tissue for Primary Cancer	colon
Case history		Metastasis
Tissue Metastasized		Genetics	colon, adenocarcinoma
Life Span		Crisis PDL
Morphology	round	Character	colon, adenocarcinoma
Classify		Established by	Semple,T.U. et.al.
Registered by	Egashira, Y.	Regulation for Distribution
Comment		Year	1987
Medium	RPMI1640 medium with 10% fetal calf serum.	Methods for Passages	Cells are treated with 0.25% trypsin.
Cell Number on Passage	2x10^4 cells/ml	Race
CO2 Conc.	5 %	Tissue Sampling	colon
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1987
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	905961

Reference
Pubmed id:14720324	Expression of VEGF-C and VEGF-D at the invasive edge correlates with lymph node metastasis and prognosis of patients with colorectal carcinoma. Onogawa S,Kitadai Y,Tanaka S,Kuwai T,Kimura S,Chayama K Cancer Sci. 2004 Jan;95(1):32-9
Pubmed id:1980764	Expression of several growth factors and their receptor genes in human colon carcinomas. Ito M,Yoshida K,Kyo E,Ayhan A,Nakayama H,Yasui W,Ito H,Tahara E Virchows Arch B Cell Pathol Incl Mol Pathol. 1990;59(3):173-8
Pubmed id:565251	Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Semple TU,Quinn LA,Woods LK,Moore GE Cancer Res. 1978 May;38(5):1345-55

Images

LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

11252003
090398
070695
12082014

Cell No.	JCRB0226	Cell Name
LOT No.	11252003	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S)	Temperature	37 C
Cell Density at Seeding	1 - 2 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA. Most of cells do not attach to substratum. Collect the non-adherent cells as wells as adherent cells at subcultivation.
Doubling Time	ca. 4 days	Cell Number in Vial (cells/1ml)	1.1 x 10^6
Viability at cell freezing (%)	87.5	Antibiotics Used	free
Passage Number	P14	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0226	Cell Name	COLO201
LOT No.	090398	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (lot#.Summit 8.1E+41).	Temperature	37 C
Cell Density at Seeding	2.0x10^4 cells/sq.cm.	Methods for Passages	Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.8 x 10^6
Viability at cell freezing (%)	80.2	Antibiotics Used	free
Passage Number	P13	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD,LD,NP examined. Human confirmed.
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	No	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO.	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0226	Cell Name
LOT No.	070695	Lot Specification	distribution
Medium	RPMI1640 + 10 % FCS	Temperature	37
Cell Density at Seeding	1/5 - 1/10	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	1.4 x 10^6
Viability at cell freezing (%)	97.2	Antibiotics Used	free
Passage Number	P12	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	AST, G6PD, LD, MPI, NP, PEPB
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0226	Cell Name	COLO201
LOT No.	12082014	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	3 x 10^5 cells/mL	Methods for Passages	0.25% trypsin and 0.02% EDTA. Most of cells do not attach to substratum. Collect the non-adherent cells as wells as adherent cells at subcultivation.
Doubling Time	approx. 4 days	Cell Number in Vial (cells/1ml)	8.0 x 10^5 cells
Viability at cell freezing (%)	84.1	Antibiotics Used	free
Passage Number	P15	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:10,13 D13S317:10,12 D7S820:9,10 D16S539:12,13 VWA:15 TH01:8,9 AM:X TPOX:11 CSF1PO:11,12
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	84.1	Additional information