-Detailed Information [JCRB0225]-

JCRB0225 COLO320 DM

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0225	Cell Name	COLO320 DM
Profile	Human colon carcinoma cell line with double minute chromosomes.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age	55
Identity	available	Tissue for Primary Cancer	colon
Case history	carcinoma of the sigmoid colon	Metastasis
Tissue Metastasized		Genetics	colon, adenocarcinoma
Life Span	infinite	Crisis PDL
Morphology	round shaped	Character	colon, adenocarcinoma
Classify	tumor	Established by	Quinn,L.A.
Registered by	Egashira, Y.	Regulation for Distribution
Comment		Year	1987
Medium	Dulbecco's modified Eagle's medium with 10% fetal calf serum.	Methods for Passages	Cells are treated with 0.25% trypsin.
Cell Number on Passage	2x10^4 cells/ml	Race	Caucasian
CO2 Conc.	5 %	Tissue Sampling	colon
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0219

Reference
Pubmed id:14720324	Expression of VEGF-C and VEGF-D at the invasive edge correlates with lymph node metastasis and prognosis of patients with colorectal carcinoma. Onogawa S,Kitadai Y,Tanaka S,Kuwai T,Kimura S,Chayama K Cancer Sci. 2004 Jan;95(1):32-9
Pubmed id:2085852	Flavonoids inhibit the expression of heat shock proteins. Hosokawa N,Hirayoshi K,Nakai A,Hosokawa Y,Marui N,Yoshida M,Sakai T,Nishino H,Aoike A,Kawai K Cell Struct Funct. 1990 Dec;15(6):393-401
Pubmed id:2479441	Multiple mechanisms in the motor responses of the guinea-pig isolated urinary bladder to bradykinin. Maggi CA,Patacchini R,Santicioli P,Geppetti P,Cecconi R,Giuliani S,Meli A Br J Pharmacol. 1989 Oct;98(2):619-29
Pubmed id:498117	Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Quinn LA,Moore GE,Morgan RT,Woods LK Cancer Res. 1979 Dec;39(12):4914-24

Images

LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

09142004
112996
03242014
091994
092094
06092021

Cell No.	JCRB0225	Cell Name
LOT No.	09142004	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S)	Temperature	37 X
Cell Density at Seeding	1.4 - 2.8 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA. Some cells grow in suspension by forming small cell aggregates. At passage, collect the floating cells as well as adherent cells.
Doubling Time	23 hrs	Cell Number in Vial (cells/1ml)	1.2 x 10^6
Viability at cell freezing (%)	96	Antibiotics Used	free
Passage Number	P10	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0225	Cell Name	COLO320 DM
LOT No.	112996	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (ICN40224155).	Temperature	37 C
Cell Density at Seeding	1-2x10^5 cells/ml	Methods for Passages	Wash cells with PBS(-) and treat them with 0.02% EDTA and 0.25% trypsin for 5 min at 37 C.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	3.4x10^6
Viability at cell freezing (%)	96.0	Antibiotics Used	free
Passage Number	P10	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:11 D7S820:9,12 D16S539:11,12 VWA:15,18 TH01:9 AM:X TPOX:8,9 CSF1PO:11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO.	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0225	Cell Name	COLO320 DM
LOT No.	03242014	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	1.7 -3.5 x 10^5 cells/mL	Methods for Passages	0.25% trypsin and 0.02% EDTA. Some cells grow in suspension by forming small cell aggregates. At passage, collect the floating cells as well as adherent cells.
Doubling Time		Cell Number in Vial (cells/1ml)	4.8 x 10^6
Viability at cell freezing (%)	93.8	Antibiotics Used	free
Passage Number	P11	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G5PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12 D13S317:11 D7S820:9,12 D16S539:11,12 VWA:15,18 TH01:9 AM:X TPOX:8,9 CSF1PO:11
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	93.8	Additional information

Cell No.	JCRB0225	Cell Name
LOT No.	091994	Lot Specification	distribution
Medium	RPMI1640 + 10 % FCS	Temperature	37
Cell Density at Seeding	1/5-1/10	Methods for Passages	0.25% trypsin and 0.02% EDTA Collecting of floating cells and attached cells
Doubling Time		Cell Number in Vial (cells/1ml)	1.1 x 10^7
Viability at cell freezing (%)	95.0	Antibiotics Used	free
Passage Number	P10	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	AST,G6PD,MPI,NP,PEPB
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0225	Cell Name
LOT No.	092094	Lot Specification	distribution
Medium	RPMI1640 + 10 % FCS Collecting of floating cells and attached cells	Temperature	37
Cell Density at Seeding	1/5-1/10	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	1.1 x 10^7
Viability at cell freezing (%)	95.0	Antibiotics Used	free
Passage Number	P10	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	AST,G6PD,MPI,NP,PEPB
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0225	Cell Name	COLO320 DM
LOT No.	06092021	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500)	Temperature	37 C
Cell Density at Seeding	4.2 - 7.7 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Some cells grow in suspension by forming small cell aggregates. At passage, collect the floating cells as well as adherent cells.
Doubling Time	16 - 28 hrs.	Cell Number in Vial (cells/1ml)	4.7 x 10^6
Viability at cell freezing (%)	89.7	Antibiotics Used	free
Passage Number	P12	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Y/N	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	86.7	Additional information