JCRB0224 WiDr

Cell information

Important Notice(s)

JCRB0224, IFO50043 WiDr is derivative from HT-29.

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0224 Cell Name   WiDr
Profile   adenocarcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   colon
Case history    Metastasis   
Tissue Metastasized    Genetics   colon, adenocarcinoma
Life Span   infinite Crisis PDL   
Morphology    Character   colon, adenocarcinoma
Classify    Established by   Wallace,R.
Registered by   Egashira, Y. Regulation for Distribution   
Comment   WiDr cell line is suggested to be derived from a human colon adenocarcinoma cell line HT-29 (Cancer Genet. Cytogenet., 27,125 (1987)) Year   1987
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.25% trypsin.
Cell Number on Passage   2x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   colon
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2760
Reference
Pubmed id:14720324Expression of VEGF-C and VEGF-D at the invasive edge correlates with lymph node metastasis and prognosis of patients with colorectal carcinoma.
Onogawa S,Kitadai Y,Tanaka S,Kuwai T,Kimura S,Chayama K
Cancer Sci. 2004 Jan;95(1):32-9
Pubmed id:698934Antiproliferative agents and differential survival between normal and cancer cells.
Tsuboi KK,Kwong LK
Cancer Res. 1978 Nov;38(11 Pt 1):3745-50

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0224 Cell Name   WiDr
LOT No.   06302010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.5-4.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.34x10^6
Viability at cell freezing (%)   96.6 Antibiotics Used   free
Passage Number   p11 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:11
D7S820:10
D16S539:11,12
VWA:17,19
TH01:6,9
AM:X
TPOX:8,9
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0224 Cell Name   
LOT No.   04172008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS Lot; GIBCO Cat. No. 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 24 hrs Cell Number in Vial (cells/1ml)   8.1 x 10^6
Viability at cell freezing (%)   97.5 Antibiotics Used   free
Passage Number   P13 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0224 Cell Name   WiDr
LOT No.   050995 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids (Gibco 27K6643) and 10% FBS. Temperature   37 C
Cell Density at Seeding   2.41x10^5 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   97.3 Antibiotics Used   free
Passage Number   P9 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0224 Cell Name   
LOT No.   040698 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1.0-1.5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   32-36 hrs Cell Number in Vial (cells/1ml)   8.4 x 10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   P3* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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