-Detailed Information [JCRB0220]-

JCRB0220 NTI-4

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0220	Cell Name	NTI-4
Profile	Normal human fibroblast.	Other Name	NTI-4
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age	9-week fetus
Identity	available	Tissue for Primary Cancer	embryo
Case history	normal	Metastasis
Tissue Metastasized		Genetics	fetus, half body, bottom/fibroblast
Life Span	finite	Crisis PDL
Morphology	fibroblast-like	Character	fetus, half body, bottom/fibroblast
Classify		Established by	Inui,N.
Registered by	Egashira,Y.	Regulation for Distribution
Comment		Year	1986
Medium	Dulbecco's modified Eagle's medium with 10% fetal calf serum	Methods for Passages	Cells are treated with 0.25% trypsin.
Cell Number on Passage	2x10^4 cells/ml	Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	fetus body
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_3080

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

01112008
042592
04162024

Cell No.	JCRB0220	Cell Name
LOT No.	01112008	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS Lot; GIBCO 484375)	Temperature	37 C
Cell Density at Seeding	4 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	approx. 20 hrs	Cell Number in Vial (cells/1ml)	7 x 10^5
Viability at cell freezing (%)	83	Antibiotics Used	free
Passage Number	P18	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), LD
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0220	Cell Name
LOT No.	042592	Lot Specification	distribution
Medium	DMDM : 10% FCS	Temperature	37
Cell Density at Seeding		Methods for Passages	0.25% trypsin
Doubling Time		Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)		Antibiotics Used
Passage Number		PDL
Sterility: MYCOPLASMA		Sterility: BACTERIA
Sterility: FUNGI		Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0220	Cell Name	NTI-4
LOT No.	04162024	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K)	Temperature	37 C
Cell Density at Seeding	4.4 - 9.1 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 1 day	Cell Number in Vial (cells/1ml)	3.6 x 10^6
Viability at cell freezing (%)	90	Antibiotics Used	free
Passage Number	P19	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:10,12 D13S317:8 D7S820:9,10 D16S539:9,12 VWA:16,19 TH01:7,8 AM:X TPOX:8 CSF1PO:10,11
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	90	Additional information