JCRB0218 CHO/HGPRT
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0218 | Cell Name | CHO/HGPRT |
|---|---|---|---|
| Profile | HGPRT deficient chinese hamster ovary (CHO) cells. | Other Name | |
| Animal | hamster, Chinese | Strain | Chinese |
| Genus | Cricetulus | Species | griseus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | ovary | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | HGPRT deficient | |
| Life Span | Crisis PDL | ||
| Morphology | Character | HGPRT deficient mutant of CHO cells | |
| Classify | Established by | Inui,N. | |
| Registered by | Egashira,Y. | Regulation for Distribution | |
| Comment | Because of the HGPRT deficient strain, the cell line is good to prepare hybridoma. | Year | 1986 |
| Medium | Ham's F12 medium with 5% fetal calf serum. | Methods for Passages | Cells are treated with 0.25% trypsin. |
| Cell Number on Passage | 2 x 10^4 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | ovary |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Images |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0218 | Cell Name | |
|---|---|---|---|
| LOT No. | 122992 | Lot Specification | distribution |
| Medium | Ham's F12 + 5% FCS + 5 ug/ml 6-thioguanine | Temperature | 37 |
| Cell Density at Seeding | 1/10-1/100 | Methods for Passages | 0.25 %trypsin and 0.02% EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | 5.0 x 10^5 | |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P6* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |