JCRB0217 DSPT200
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0217 | Cell Name | DSPT200 |
|---|---|---|---|
| Profile | fetus fibroblast transformed cell | Other Name | DSPT200 |
| Animal | hamster, Syrian | Strain | Syrian |
| Genus | Mesocricetus | Species | auratus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | embryo | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | fetus fibroblast transformed cell | |
| Life Span | Crisis PDL | ||
| Morphology | Character | fetus fibroblast transformed cell | |
| Classify | Established by | Inui,N. | |
| Registered by | Egashira,Y. | Regulation for Distribution | |
| Comment | Year | 1986 | |
| Medium | Eagle's minimal essential medium with 10% fetal calf serum. | Methods for Passages | Cells are treated with 0.25% trypsin. |
| Cell Number on Passage | 2x10^4 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | fetus |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0217 | Cell Name | DSPT200 |
|---|---|---|---|
| LOT No. | 03242000 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum (Intergen RB52405). | Temperature | 37 C |
| Cell Density at Seeding | 1.6 x 10^4 cells/sq.cm. | Methods for Passages | Harvest cells after treatment with 0.25% trypsin and 0.02% EDTA at room temperature for 2.5 minutes. Subculture once a week. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.3 x 10^6 |
| Viability at cell freezing (%) | 97.0 | Antibiotics Used | free |
| Passage Number | P3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD, LD, and NP were examined. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cells frozen in the fresh culture medium containing 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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