JCRB0213 HeLa AG

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0213 Cell Name   HeLa AG
Profile   epitheloid carcinoma, 8-azaguanine-resistant Other Name   HeLa AG
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   31
Identity   available Tissue for Primary Cancer   uterine cervix
Case history    Metastasis   
Tissue Metastasized    Genetics   cervix, AG-r
Life Span    Crisis PDL   
Morphology   epithelial-like Character   cervix, AG-r
Classify    Established by   Sekiguchi,T.
Registered by   Isaka,H. Regulation for Distribution   Report to Dr.Sekiguchi is required.
Comment   Resistant to 8-azaguanine. Year   1985
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.25% trypsin.
Cell Number on Passage   2 x 10^4 cells/ml Race   black
CO2 Conc.   5 % Tissue Sampling   cervix
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 +
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2933
Reference
Pubmed id:9892199Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping.
Macville M,Schröck E,Padilla-Nash H,Keck C,Ghadimi BM,Zimonjic D,Popescu N,Ried T
Cancer Res. 1999 Jan 1;59(1):141-50
Pubmed id:2754009Comparison of HeLa 229 and McCoy cell cultures for detection of Chlamydia trachomatis in clinical specimens.
Thewessen EA,Freundt I,van Rijsoort-Vos JH,Stolz E,Michel MF,Wagenvoort JH
J Clin Microbiol. 1989 Jun;27(6):1399-400
Pubmed id:3145877Enhancement of yield of Chlamydia trachomatis Hela 229 cell culture.
Wagenvoort JT,van Rijsoort-Vos T,Overkleeft-van de Ree A,Stolz E
Eur J Clin Microbiol Infect Dis. 1988 Dec;7(6):822
Pubmed id:14185313ANIMAL CELL STRAINS. THE CELL CULTURE COLLECTION COMMITTEE HAS ASSEMBLED AND CERTIFIED 23 STRAINS OF ANIMAL CELLS.
LEDLEY RS
Science. 1964 Oct 9;146(3641):241-3
Pubmed id:13237281Preservation at subzero temperatures of mouse fibroblasts (strain L) and human epithelial cells (strain HeLa).
SCHERER WF,HOOGASIAN AF
Proc Soc Exp Biol Med. 1954 Nov;87(2):480-7

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0213 Cell Name   HeLa AG
LOT No.   08152000 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   1.7-2.1x10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA at room temperature for 5 minutes. Subculture twice a week and change medium every 1-2 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   95.4 Antibiotics Used   free
Passage Number   P10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeA), LDH and NP examined, by Sasaki,A..
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:14
D7S820:8
D16S539:9,10
VWA:16,18
TH01:7
AM:X
TPOX:12
CSF1PO:9,10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0213 Cell Name   HeLa AG
LOT No.   020389 Lot Specification   distribution
Medium   MEM : 10%FCS Temperature   37 C
Cell Density at Seeding   2 x 10^4 cells/ml Methods for Passages   0.25% Trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.5x10^6
Viability at cell freezing (%)   93% Antibiotics Used   free
Passage Number   P13* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,G6PD,NP,MD,PEPB,AST
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:13.3
D7S820:8
D16S539:9,10
VWA:16,18
TH01:7
AM:X
TPOX:12
CSF1PO:9,10
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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