JCRB1077 DD-762

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1077 Cell Name   DD-762
Profile   Mouse mammary tumor cell line producing MuMTV. Other Name   
Animal   mouse Strain   DD/Tbr
Genus   Mus Species   musculus
Sex   F Age   adult
Identity   not done Tissue for Primary Cancer   mammary
Case history   normal but spontaneous tumor arisen. Metastasis   
Tissue Metastasized    Genetics   MuMTV is positive.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   DD-762 was isolated directly from DD-761 established from the mouse mammary tumor. Td=80. Modal No. of chromosome=80.
Classify   tumor Established by   Iwai,Y.
Registered by   Iwai,M. Regulation for Distribution   
Comment    Year   2003
Medium   Dulbecco's modified Eagle's medium(4.5g/L glucose) with 10% fetal calf serum and 10mg/L insulin. Methods for Passages   Cells harvested after treatment with trypsin-EDTA.
Cell Number on Passage   not defined Race   
CO2 Conc.   5 % Tissue Sampling   tumor tissue
Tissue Type   mammary tumor

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8185
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1077 Cell Name   
LOT No.   06232005 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (4.5 g/L glucose) with 10 mg/L insulin and 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   21 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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