JCRB1074 FU97

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1074 Cell Name   FU97
Profile   Human stomach cancer cell line with AFP production. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   66
Identity   available Tissue for Primary Cancer   stomach
Case history   stomach cancer, 2266ng/ml AFP observed before the operation. Lymph node metastasis and pancreatic metastasis observed. ss, ssINFbehta, ly3, v0. Metastasis   Yes
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Td = 95 hrs. AFP production and tumorigenesity is maintained in the cell line.
Classify   tumor Established by   Matsuda,M.
Registered by   Iwai,M. Regulation for Distribution   
Comment    Year   2003
Medium   Dulbecco's modified Eagle's medium(4.5g/L glucose) with 10% fetal calf serum and 10mg/L insulin. Methods for Passages   Cells are harvested after treatment with trypsin-EDTA.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   stomach
Tissue Type   poorly differentiated adenocarcinoma.

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2908
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1290806
Reference
Pubmed id:10614861Establishment of an alpha-fetoprotein-producing cell line derived from gastric cancer.
Matsuda M,Watanabe A,Sawada H,Yamada Y,Nakano H,Iwai M,Iwai Y
In Vitro Cell Dev Biol Anim. 1999 Nov-Dec;35(10):555-7
Pubmed id:noneBiological behavior of an alpha-fetoprotein-producing gastric cancer cell line (FU97)
Matsuda, M.
J. Nara Med. Ass., 51: 79-89 (2000)

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1074 Cell Name   FU97
LOT No.   02022011 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose; 4.5 g/L) with 10 mg/L insulin and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.4 - 2.2 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 34 hrs. Cell Number in Vial (cells/1ml)   9.3 x 10^5
Viability at cell freezing (%)   73.8 Antibiotics Used   free
Passage Number   Unknown (12 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1074 Cell Name   
LOT No.   08072007 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (4.5g/L glucose) with 10% fetal calf serum (lot:GIBCO484375) and 10mg/L insulin. Temperature   37C
Cell Density at Seeding   2.0 x 10^4cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS
Doubling Time   30hr Cell Number in Vial (cells/1ml)   7.5 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   P12* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   70% Culture medium + 20% FCS + 10% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1074 Cell Name   FU97
LOT No.   02052004 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (High glucose) with 10% fetal calf serum (Intergen RB52305) and 10mg/L insulin. Temperature   37 C
Cell Density at Seeding   8.8 x 10^3 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.3% trypsin. Subculture once 5-7 days and medium change every 2-3 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   97.9 Antibiotics Used   free
Passage Number   P9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5 % DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1074 Cell Name   FU97
LOT No.   03132015 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (4.5g/L glucose) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) and 10 mg/L insulin. Temperature   37 C
Cell Density at Seeding   1.3 - 1.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (typeB), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,12
D13S317:10,12
D7S820:11
D16S539:9,11
VWA:17
TH01:9
AM:X
TPOX:12
CSF1PO:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   95 Additional information   
Cell No.   JCRB1074 Cell Name   FU97
LOT No.   02192019 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (4.5 g/L glucose) with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) and 10 mg/L insulin Temperature   37 C
Cell Density at Seeding   2.0 - 2.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 41 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   90.3 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
Viability immediately after thawing (%)   90.3 Additional information   
Cell No.   JCRB1074 Cell Name   FU97
LOT No.   06122023 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322) and 10 mg/L insulin Temperature   37 C
Cell Density at Seeding   1.8 - 2.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 36 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,12
D13S317:10,12
D7S820:11
D16S539:9,11
VWA:17
TH01:9
AM:X
TPOX:12
CSF1PO:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
Viability immediately after thawing (%)   83 Additional information   
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