-Detailed Information [JCRB1066]-

JCRB1066 KMH-2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB1066	Cell Name	KMH-2
Profile	Human anaplasitc thyroid carcinoma cell line with multidrug resistance, derived from a left cervical tumor with a sligt leukocytosis.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	71
Identity	available	Tissue for Primary Cancer	thyroid
Case history	left cervical tumor with a slight leukocytosis, a biopsy revealed an anaplastic thyroid.	Metastasis
Tissue Metastasized		Genetics	Chromosome No. 92-108.
Life Span	infinite	Crisis PDL
Morphology	spindle shaped cells with giant cells	Character	doubling time is 58 hours and multidrug registance.
Classify	tumor	Established by	Sekiguchi,K. et. al.
Registered by	Sekiguchi,K.	Regulation for Distribution	no
Comment		Year	2003
Medium	1 to 1 mixture of Dulbecco's modified Eagle's medium and RPMI1640 medium with 10% heat inactivated feta; bovine serum.	Methods for Passages	Cells are harvested after treatment with 0.25% trypsin.
Cell Number on Passage	10^4 cell/ml	Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	cervical tumor
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_S641
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	1298167

Reference
Pubmed id:11686581	Biological characteristics and chemosensitivity profile of four human anaplastic thyroid carcinoma cell lines. Sekiguchi M,Shiroko Y,Arai T,Kishino T,Sugawara I,Kusakabe T,Suzuki T,Yamashita T,Obara T,Ito K,Hasumi K Biomed Pharmacother. 2001 Oct;55(8):466-74

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

01302004
04192022

Cell No.	JCRB1066	Cell Name
LOT No.	01302004	Lot Specification	distribution
Medium	1 : 1 mixture of Dulbecco's modified Eagle's medium and RPMI1640 medium with 10% heat-inactivated fetal bovine serum (FBS Lot; GIBCO 3762782S)	Temperature	37 C
Cell Density at Seeding	4 - 10 x 10^4 cells/ml	Methods for Passages	0.25% trypsin
Doubling Time	1.1 - 1.5 days	Cell Number in Vial (cells/1ml)	7.4 x 10^5
Viability at cell freezing (%)	93.7	Antibiotics Used	free
Passage Number	P278	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% heat-inactivated FBS in DMEM:RPMI1640 = 1:1	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1066	Cell Name	KMH-2
LOT No.	04192022	Lot Specification	distribution
Medium	1 : 1 mixture of Dulbecco's modified Eagle's medium (GIBCO,Cat No.11885-084) and RPMI1640 medium ( GIBCO,Cat No.11875-093) with 10% heat-inactivated fetal bovine serum (Biowest cat.No.S1820-500 LotNo.S09336S1820)	Temperature	37 C
Cell Density at Seeding	1.07.-6.61x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.41x10^6
Viability at cell freezing (%)	94.7	Antibiotics Used	free
Passage Number	p279	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12,13 D13S317:9 D7S820:11 D16S539:9,12 VWA:14,15 TH01:9 AM:X,Y TPOX:8,11 CSF1PO:10,11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	90.7	Additional information

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