JCRB1065 Ki-JK

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1065 Cell Name   Ki-JK
Profile   Human mononuclear cells with multiple nucleoli from lymphoma patient. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   15
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   diagnosed as Ki-1 positive lymphoma in patient with tumor in the inguinal region including skin. Metastasis   
Tissue Metastasized    Genetics   Positive markers, CD15,CD25,CD30,CD45,CD45RO,EMA,Macrophage,HLA-ABC,HLA-DR,Tf-R,Ki-6,Anti-lysozyme
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   Mononuclear cells with multiple nucleoli. No growth in soft agar.
Classify   tumor Established by   Shimakage,M.
Registered by   Shimakage,M. Regulation for Distribution   
Comment   The STR-PCR pattern indicated to be a unique cell line. Year   2003
Medium   RPMI1640 medium with 10% fetal calf serum with 5% CO2. Subculture twice a week. Methods for Passages   simple dilution but do not dilute extensively, over 1 x 10^6 cells/ml.
Cell Number on Passage   > 1 x 10^6 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   pleural fluid
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2093
Reference
Pubmed id:8169113A Ki-1-positive cell line expressing Epstein-Barr virus antigens, established from a child with Ki-1-positive lymphoma.
Shimakage M,Dezawa T,Tamura S,Tabata T,Aoyagi N,Koike M,Inoue H,Yutsudo M,Hakura A,Ikegami N
Intervirology. 1993;36(4):215-24

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1065 Cell Name   
LOT No.   11072012 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099). Use of 20% FBS is recommended during several days after thawing. Temperature   37 C
Cell Density at Seeding   2.1 - 3.3 x 10^5 cells/mL Methods for Passages   Dilution (suspension culture)
Doubling Time   approx. 83 hrs Cell Number in Vial (cells/1ml)   9.3 x 10^5
Viability at cell freezing (%)   83.8 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1065 Cell Name   
LOT No.   08112004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   Cells were harvested by pipetting. Subculture twice a week.
Doubling Time   ca. 24 hrs Cell Number in Vial (cells/1ml)   9.5 x 10^5
Viability at cell freezing (%)   82.1 Antibiotics Used   free
Passage Number   P7 at cellbank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1065 Cell Name   Ki-JK
LOT No.   07292003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum (S, IntergenRB52305). Temperature   37 C
Cell Density at Seeding   2.0 x 10^5 cells/ml Methods for Passages   simple dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   89.3 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1065 Cell Name   Ki-JK
LOT No.   11212011 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA Cat#F0926, lot 027K03911) Temperature   37 C
Cell Density at Seeding   1.4-3.8x10^5 cells/ml Methods for Passages   simple dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.79x10^6
Viability at cell freezing (%)   89.7 Antibiotics Used   free
Passage Number   p11* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:9,12
D7S820:10,12
D16S539:10
VWA:17,18
TH01:8,9
AM:X,Y
TPOX:10,11
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
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