JCRB0208 CCK-81

Cell information

Important Notice(s)

JCRB0208 CCK-81 : Notation for culturing

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0208 Cell Name   CCK-81
Profile   adenocarcinoma Other Name   CCK-81
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   colon
Case history   adenocarcinoma Metastasis   
Tissue Metastasized    Genetics   large intestine
Life Span   infinite Crisis PDL   
Morphology    Character   carcinoembryonic antigen producing.
Classify   tumor Established by   Koura,M.
Registered by   Isaka,H. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.25 % trypsin.
Cell Number on Passage   2x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   colon
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2873
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1240123
Reference
Pubmed id:2454734Selection of a monoclonal antibody reactive with a high-molecular-weight glycoprotein circulating in the body fluid of gastrointestinal cancer patients.
Sakurai Y,Hirohashi S,Shimosato Y,Kodaira S,Abe O
Cancer Res. 1988 Jul 15;48(14):4053-8
Pubmed id:7418977Establishment of a human colon adenocarcinoma cell line producing carcinoembryonic antigen.
Koura M,Isaka H
Gan. 1980 Jun;71(3):313-8

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0208 Cell Name   
LOT No.   03032010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   0.5 - 1.3 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage the cells if the cultures reach to 50% confluency.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   82.9 Antibiotics Used   free
Passage Number   P11 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0208 Cell Name   
LOT No.   020498 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1-5 x 10^5 cells/ml Methods for Passages   0.25% trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   3.9 x 10^6
Viability at cell freezing (%)   86.0 Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0208 Cell Name   
LOT No.   090393 Lot Specification   distribution
Medium   MEM :10 % FCS Temperature   37
Cell Density at Seeding   2x10^4/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0208 Cell Name   CCK-81
LOT No.   03182016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.7 - 1.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 40 hrs. Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   69.3 Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12,13
D13S317:9,12,13
D7S820:8,8.1,10.3,12
D16S539:9,10
VWA:14,18
TH01:6,8
AM:X
TPOX:9,11
CSF1PO:10,13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   69.3 Additional information   
Cell No.   JCRB0208 Cell Name   CCK-81
LOT No.   11012017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   6 - 8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   2 - 3 days Cell Number in Vial (cells/1ml)   9.9 x 10^5
Viability at cell freezing (%)   83.9 Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   83.9 Additional information   
Cell No.   JCRB0208 Cell Name   CCK-81
LOT No.   02202023 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   2.1 - 4.6 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage the cells when the cultures reach to 50% confluency.
Doubling Time   approx. 35 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P11 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12,13
D13S317:9,12,13
D7S820:8,8.1,10.3,12
D16S539:9,10
VWA:14,18
TH01:6,8
AM:X
TPOX:9,11
CSF1PO:10,13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   45.7 Additional information   
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