JCRB0114 LC4-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0114 Cell Name   LC4-1
Profile   leukemia, acute non T lymphoblastic Other Name   LC4-1
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   13
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute non-T cell lymphoblastic leukemia Metastasis   No
Tissue Metastasized    Genetics   positive Ia,B4, negative B2,Tac,T3,T4,T8,T11 & M1
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   E-rossette formation. D-J joinning. EBNA negative.
Classify   tumor Established by   Mayumi,M.
Registered by   Mayumi,M. Regulation for Distribution   Not regulated.
Comment    Year   
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   dilute 1/10 every 3-4 day
Cell Number on Passage   1-2 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1374
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907787
Reference
Pubmed id:3115091Establishment of a common acute lymphoblastic leukemia cell line (LC4-1) and effects of phorbol myristate acetate (PMA) on the surface antigen expression of the cell line.
Yoshimura T,Mayumi M,Yorifuji T,Kim KM,Heike T,Miyanomae T,Shinomiya K,Mikawa H
Am J Hematol. 1987 Sep;26(1):47-54

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0114 Cell Name   
LOT No.   06122002 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time    Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (P13 at cellbank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS in RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0114 Cell Name   LC4-1
LOT No.   051691 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37 C
Cell Density at Seeding   1-2x10^6 cells/10cm dish Methods for Passages   dilution 1/10 every 3-4 days
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.6x10^5
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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