JCRB0104.2 KU812F

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0104.2 Cell Name   KU812F
Profile   leukemia, chronic myeloid Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   38
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   chronic myeloid leukemia (CML) Metastasis   
Tissue Metastasized    Genetics   Ph1 chr., histamin(+),t(9;22)(q34;q11),i(17q) etc.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Dt=20-30hr, clonal isolation of KU812
Classify   tumor Established by   Kishi,K.
Registered by   Kishi,K. Regulation for Distribution   
Comment   This cell line was deposited to ATCC, ECACC and DSM at 1990. Year   1987
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Simple dilution.
Cell Number on Passage   10^5 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0616
Reference
Pubmed id:2540861KU 812: a pluripotent human cell line with spontaneous erythroid terminal maturation.
Nakazawa M,Mitjavila MT,Debili N,Casadevall N,Mayeux P,Rouyer-Fessard P,Dubart A,Roméo PH,Beuzard Y,Kishi K
Blood. 1989 May 15;73(7):2003-13
Pubmed id:3304457Bipotential cell differentiation of KU-812: evidence of a hybrid cell line that differentiates into basophils and macrophage-like cells.
Fukuda T,Kishi K,Ohnishi Y,Shibata A
Blood. 1987 Sep;70(3):612-9
Pubmed id:2436686A monoclonal antibody reacting with human basophils.
Bodger MP,Mounsey GL,Nelson J,Fitzgerald PH
Blood. 1987 May;69(5):1414-8
Pubmed id:3695564Induction of differentiation of the human leukemia cell line, KU812.
Fischkoff SA,Kishi K,Benjamin WR,Rossi RM,Hoessly MC,Hoxie JA,Seals C,Anderson L,Ishizaka T,Hakimi J
Leuk Res. 1987;11(12):1105-13
Pubmed id:3858609A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors.
Kishi K
Leuk Res. 1985;9(3):381-90

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0104.2 Cell Name   KU812F
LOT No.   081194 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat-inactivated fetal bovine serum (J.R.Scientific, DO10400) Temperature   37 C
Cell Density at Seeding   3-5x10^5 cells/ml Methods for Passages   Simple dilution.
Doubling Time   38.5 hrs Cell Number in Vial (cells/1ml)   5.7x10^6
Viability at cell freezing (%)   71.9 Antibiotics Used   free
Passage Number   P9* PDL   NT
Sterility: MYCOPLASMA   + Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   nT DNA Profile (STR)   D5S818:10
D13S317:8
D7S820:12
D16S539:9
VWA:15
TH01:9
AM:XY
TPOX:12
CSF1PO:12,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0104.2 Cell Name   KU812F
LOT No.   081490 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0104.2 Cell Name   KU812F
LOT No.   061890 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.5x10^5
Viability at cell freezing (%)   83% Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0104.2 Cell Name   KU812F
LOT No.   113087 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   84% Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0104.2 Cell Name   KU812F
LOT No.   06092015 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   3.6 x 10^5 cells/ml Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 37 hrs Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10
D13S317:8
D7S820:12
D16S539:9
VWA:15
TH01:9
AM:X,Y
TPOX:12
CSF1PO:12,13
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   85 Additional information   
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