JCRB0103 VMRC-LCP

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0103 Cell Name   VMRC-LCP
Profile   squamous cell carcinoma Other Name   Patton
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   lung squamous cell carcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   lung squamous cell carcinoma
Classify   tumor Established by   Bean,M.A.
Registered by   Akiyama,M. Regulation for Distribution   not defined
Comment    Year   1986
Medium   Eagle's minimal essential medium with non essensial amino acids and 10% fetal calf serum Methods for Passages   0.02% EDTA and 0.25% trypsin. Scraping is better than trypsinization because of high plating efficiency.
Cell Number on Passage   split ratio=1/6 - 1/8 Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1788
Reference
Pubmed id:2164440Potent growth inhibition of human tumor cells in culture by arginine deiminase purified from a culture medium of a Mycoplasma-infected cell line.
Miyazaki K,Takaku H,Umeda M,Fujita T,Huang WD,Kimura T,Yamashita J,Horio T
Cancer Res. 1990 Aug 1;50(15):4522-7
Pubmed id:2736210Production of transforming growth factor-alpha in human tumour cell lines.
Imanishi K,Yamaguchi K,Suzuki M,Honda S,Yanaihara N,Abe K
Br J Cancer. 1989 May;59(5):761-5

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0103 Cell Name   VMRC-LCP
LOT No.   101196 Lot Specification   distribution
Medium   Eagle's MEM with non-esseitial amino acids and 10%fetal calf serum (FCS lot; JRH Biosci. 1B1062) Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   Wash with PBS(-) and treat with 0.25% trypsin and 0.02% EDTA for 15 min at 37 C.
Doubling Time    Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   P49 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   95.0 Additional information   
Images
Cell No.   JCRB0103 Cell Name   VMRC-LCP
LOT No.   082994 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential AAs and 10% fetal bovine serum (JRS.D010400). Temperature   37 C
Cell Density at Seeding   2-2.5x10^4 cells/sq.cm Methods for Passages   Wash cells once with PBS(-) and treated with 0.1% trypsin and 0.01% EDTA for 13-16 min. at 37 C.
Doubling Time   93.0 hr Cell Number in Vial (cells/1ml)   1.48x10^6
Viability at cell freezing (%)   98.2 Antibiotics Used   free
Passage Number   P52 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),LD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0103 Cell Name   VMRC-LCP
LOT No.   061792 Lot Specification   distribution
Medium   E.MEM+NEAA + 10% FCS Temperature   37C
Cell Density at Seeding   6x10^5 cells /ml Methods for Passages   Scraping or 0.02%EDTA-0.05%trypsin treatment. Suspend in 2 ml of the medium.EDTA-trypsin recomended.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.7x10^5
Viability at cell freezing (%)   97% Antibiotics Used   free
Passage Number   P50 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0103 Cell Name   VMRC-LCP
LOT No.   112087 Lot Specification   distribution
Medium   MEM w/ non essensial amino acids + FCS 10% Temperature   37
Cell Density at Seeding   split ratio:1/6 - 1/8 Methods for Passages   0.01% or 0.02% EDTA and 0.05% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P49 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0103 Cell Name   VMRC-LCP
LOT No.   11132014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essensial amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.7 - 2.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.05 - 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 79 hrs Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P56 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM-NEAA CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
Cell No.   JCRB0103 Cell Name   VMRC-LCP
LOT No.   05312022 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   4.9 x 10^6
Viability at cell freezing (%)   92.5 Antibiotics Used   free
Passage Number   P54 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D7S820:10,12
D16S539:12
VWA:18,20
TH01:7
AM:X
TPOX:8
CSF1PO:10
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   96.1 Additional information   
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