JCRB1044 OVKATE

Cell information

Important Notice(s)

The growth property of OVKATE (JCRB1044): Growth is very slow.

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1044 Cell Name   OVKATE
Profile   Human cell line derived from ovarian tumor. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   40
Identity   available Tissue for Primary Cancer   ovary
Case history   ovarian carcinoma, stage IIIc, CAP(3) and EP(3) treatment done previously. Metastasis   No
Tissue Metastasized    Genetics   ER(6.2 fmol/mg), PR(-), tumor markers under investigation.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cells are transplantable to both mouse hypodermis and abdominal.
Classify   tumour Established by   Gorai,I. et.al.
Registered by   Gorai,I. Regulation for Distribution   
Comment   Td=18.3 hours Year   2002
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells are harvested after treatment with 0.05% trypsin. Change medium every 3-4 days.
Cell Number on Passage   1 x 10^5 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   ovary
Tissue Type   adeno carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3110
Reference
Pubmed id:11121861Cyclophosphamide and 5-fluorouracil act synergistically in ovarian clear cell adenocarcinoma cells.
Ohta I,Gorai I,Miyamoto Y,Yang J,Zheng JH,Kawata N,Hirahara F,Shirotake S
Cancer Lett. 2001 Jan 10;162(1):39-48
Pubmed id:9328139Complexity of expression of the intermediate filaments of six new human ovarian carcinoma cell lines: new expression of cytokeratin 20.
Yanagibashi T,Gorai I,Nakazawa T,Miyagi E,Hirahara F,Kitamura H,Minaguchi H
Br J Cancer. 1997;76(7):829-35
Pubmed id:7535723Establishment and characterization of two human ovarian clear cell adenocarcinoma lines from metastatic lesions with different properties.
Gorai I,Nakazawa T,Miyagi E,Hirahara F,Nagashima Y,Minaguchi H
Gynecol Oncol. 1995 Apr;57(1):33-46
Pubmed id:7882618Marked induction of gelatinases, especially type B, in host fibroblasts by human ovarian cancer cells in athymic mice.
Miyagi E,Yasumitsu H,Hirahara F,Nagashima Y,Minaguchi H,Miyazaki K,Umeda M
Clin Exp Metastasis. 1995 Mar;13(2):89-96

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1044 Cell Name   
LOT No.   04302008 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   1 - 4 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   very slow. D.T. = 5 - 6 days Cell Number in Vial (cells/1ml)   6.7 x 10^6
Viability at cell freezing (%)   94.3 Antibiotics Used   free
Passage Number   P45 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1044 Cell Name   OVKATE
LOT No.   05122003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   2.0 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.05% trypsin. Subculture once every 10-20 days. Medium change every 3 to 4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   96.8 Antibiotics Used   NT
Passage Number   NT PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human, not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   available
Medium for Freezing   NT CO2 Conc.   NT
Viability immediately after thawing (%)   NT Additional information   
Images
Cell No.   JCRB1044 Cell Name   OVKATE
LOT No.   05232014 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   1.2 - 3.4 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   7.1 x 10^6
Viability at cell freezing (%)   83.3 Antibiotics Used   free
Passage Number   P44 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,13
D13S317:12
D7S820:11,12
D16S539:9
VWA:18
TH01:9
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   83.3 Additional information   Doubling time was 5 - 7 days (very slow). Expected viability (defrost ampoule) 88 %.
Cell No.   JCRB1044 Cell Name   OVKATE
LOT No.   05132016 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   2.1 - 2.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Growth is very slow.
Doubling Time   very slow Cell Number in Vial (cells/1ml)   5.7 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   P49 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   91 Additional information   
Cell No.   JCRB1044 Cell Name   OVKATE
LOT No.   12042017 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   slow (> 1 week) Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   P45 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   85 Additional information   
Cell No.   JCRB1044 Cell Name   OVKATE
LOT No.   11112020 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.7 - 2.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   very slow (approx. 6 days) Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P47 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   79 Additional information   
Cell No.   JCRB1044 Cell Name   OVKATE
LOT No.   04072025 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   2 - 5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.5% trypsin and 0.02% EDTA.
Doubling Time   Slow (5-6 days) Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   92.0 Antibiotics Used   free
Passage Number   P47 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   79.7 Additional information   
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