JCRB1043 OVISE

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1043 Cell Name   OVISE
Profile   Human cell line derived from ovarian tumor. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   40
Identity   available Tissue for Primary Cancer   ovary
Case history   ovarian carcinoma, stage IIb, CAP(3) treatment done previously. Metastasis   Yes
Tissue Metastasized   innominate bone Genetics   ER(-), PR(-) / tumour markers, CA19-9, CA125, TPA.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cells are transpantable on mouse hypodermic but not abdominal.
Classify   tumour Established by   Nakazawa,T.
Registered by   Gorai,I. Regulation for Distribution   
Comment    Year   2002
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells are harvested after treatment with 0.05% trypsin. Change medium every 3-4 days.
Cell Number on Passage   5 x 10^4 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   metastatic forcus of the innominate
Tissue Type   clear cell adeno carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3116
Reference
Pubmed id:11121861Cyclophosphamide and 5-fluorouracil act synergistically in ovarian clear cell adenocarcinoma cells.
Ohta I,Gorai I,Miyamoto Y,Yang J,Zheng JH,Kawata N,Hirahara F,Shirotake S
Cancer Lett. 2001 Jan 10;162(1):39-48
Pubmed id:9328139Complexity of expression of the intermediate filaments of six new human ovarian carcinoma cell lines: new expression of cytokeratin 20.
Yanagibashi T,Gorai I,Nakazawa T,Miyagi E,Hirahara F,Kitamura H,Minaguchi H
Br J Cancer. 1997;76(7):829-35
Pubmed id:7535723Establishment and characterization of two human ovarian clear cell adenocarcinoma lines from metastatic lesions with different properties.
Gorai I,Nakazawa T,Miyagi E,Hirahara F,Nagashima Y,Minaguchi H
Gynecol Oncol. 1995 Apr;57(1):33-46
Pubmed id:7882618Marked induction of gelatinases, especially type B, in host fibroblasts by human ovarian cancer cells in athymic mice.
Miyagi E,Yasumitsu H,Hirahara F,Nagashima Y,Minaguchi H,Miyazaki K,Umeda M
Clin Exp Metastasis. 1995 Mar;13(2):89-96
Pubmed id:noneEstablishment and characterization of human ovarian clear cell carcinoma cell line, OVISE.
Nakazawa T., Gorai I., Doi C., Hirahara F., Minaguchi H.
J Jpn Soc Clin Cytol. 1992 Nov; 31(6):966-72

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1043 Cell Name   OVISE
LOT No.   06132011 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099). Temperature   37 C
Cell Density at Seeding   0.7 - 2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   92.3 Antibiotics Used   free
Passage Number   P37 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10
D13S317:11,12
D7S820:11,12
D16S539:9
VWA:18
TH01:9,9.3
AM:X
TPOX:8
CSF1PO:9,11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1043 Cell Name   
LOT No.   04092007 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   8-16 x 10^4 cells/ml Methods for Passages   0.05% trypsin
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   92.7 Antibiotics Used   free
Passage Number   P37 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by G6PD (typeB), NP, LD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1043 Cell Name   OVISE
LOT No.   05302016 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   1.3 - 1.8 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA.
Doubling Time   approx. 49 hrs Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   96.5 Antibiotics Used   free
Passage Number   P37 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   96.5 Additional information   
Cell No.   JCRB1043 Cell Name   OVISE
LOT No.   03022020 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.5 - 1.4 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 30 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   P37 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   92 Additional information   
Cell No.   JCRB1043 Cell Name   OVISE
LOT No.   07042022 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322) Temperature   37 C
Cell Density at Seeding   7-8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.05% trypsin solution.
Doubling Time   approx. 40 hrs. Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P36 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10
D13S317:11,12
D7S820:11,12
D16S539:9
VWA:18
TH01:9,9.3
AM:X
TPOX:8
CSF1PO:9,11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS (GIBCO Cat. # 10437, Lot 1072322) - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   92 Additional information   
Images
Cell No.   JCRB1043 Cell Name   OVISE
LOT No.   10212024 Lot Specification   distribution
Medium   RPMI1640(GIBCO Cat #11875-093) with 10% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   1.3-1.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin in PBS.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   p36 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:11,12
D7S820:11,12
D16S539:9
VWA:18
TH01:9,9.3
AM:X
TPOX:8
CSF1PO:9,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   94 Additional information   
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