-Detailed Information [JCRB1049]-

JCRB1047 OVSAYO、JCRB1049 OVMIU、JCRB1050 OVMIU-IIに関するご注意

OVSAYO細胞は、64歳女性、ステージIc 卵巣がん(腺がん)に由来する細胞として樹立されましたが、JCRBの調査により、他の卵巣がん由来細胞であるOVMIU細胞(46歳女性、ステージIIIc, 腺がん)(JCRB1049)と同一のDNAパターンを示すことが明らかになっております。一方、OVMIU細胞と同一患者に由来するとされるOVMIU-II細胞(JCRB1050)は、OVMIU細胞とは異なるDNAパターンを示します。樹立者の研究室に保管されているもっとも継代数の若いストックについても同様の結果が得られています。OVMIU細胞の樹立の初期にOVSAYOが混入、置換してしまった可能性と、OVMIU-IIとOVSAYO細胞が入れ替わってしまっている可能性等があります。これら細胞株の由来する個体については明確でないことをご承知の上ご利用くださいますようお願い申し上げます。

Problem of JCRB1047 OVSAYO、JCRB1049 OVMIU、JCRB1050 OVMIU-II

OVSAYO was reported to be established from ovarian adenocarcinoma stage Ic, 64-year-old female patient. However, DNA-based analysis in JCRB cell bank revealed that OVSAYO is identical to the other ovarian cancer cell line, OVMIU (adenocarcinoma stage IIIc, 46-year-old patient). In addition OVMIU-II was reported to be derived from the same patient as OVMIU but the DNA pattern is not identical to OVMIU. The same result was obtained for the lots of cell lines with younger passages preserved in the developer's laboratory. Apparently, cross-culture contamination or misplacement occured during the establishment or maintenance of these cell lines. Therefore please notice that the identities of these cell lines are ambiguous.
 

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JCRB No.	JCRB1049	Cell Name	OVMIU
Profile	Human cell line derived from ovarian tumour.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age	46
Identity	available	Tissue for Primary Cancer	ovary
Case history	ovarian carcinoma, stage IIIc, not treated before.	Metastasis	No
Tissue Metastasized		Genetics	ER(-) / tumor markers, CA125, CA19-9, TAP, SLX, LDH, ALP E2, E1, DHEA.
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	Cells are not transplantable to mouse.
Classify	tumour	Established by	Gorai,I. et.al.
Registered by	Gorai,I.	Regulation for Distribution
Comment	The STR pattern of the OVMIU was consistent with the OVSAYO while the OVMIU-II was expected to be same with the OVMIU.	Year	2002
Medium	RPMI1640 medium with 10% fetal bovine serum.	Methods for Passages	Cells are harvested after treatment with 0.05% trypsin. Change medium every 3-4 days.
Cell Number on Passage	5 x 10^4 cells/ml	Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	ovary
Tissue Type	adeno carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_3112
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	1240200

Reference
Pubmed id:11121861	Cyclophosphamide and 5-fluorouracil act synergistically in ovarian clear cell adenocarcinoma cells. Ohta I,Gorai I,Miyamoto Y,Yang J,Zheng JH,Kawata N,Hirahara F,Shirotake S Cancer Lett. 2001 Jan 10;162(1):39-48
Pubmed id:9328139	Complexity of expression of the intermediate filaments of six new human ovarian carcinoma cell lines: new expression of cytokeratin 20. Yanagibashi T,Gorai I,Nakazawa T,Miyagi E,Hirahara F,Kitamura H,Minaguchi H Br J Cancer. 1997;76(7):829-35
Pubmed id:7535723	Establishment and characterization of two human ovarian clear cell adenocarcinoma lines from metastatic lesions with different properties. Gorai I,Nakazawa T,Miyagi E,Hirahara F,Nagashima Y,Minaguchi H Gynecol Oncol. 1995 Apr;57(1):33-46
Pubmed id:7882618	Marked induction of gelatinases, especially type B, in host fibroblasts by human ovarian cancer cells in athymic mice. Miyagi E,Yasumitsu H,Hirahara F,Nagashima Y,Minaguchi H,Miyazaki K,Umeda M Clin Exp Metastasis. 1995 Mar;13(2):89-96

JCRB Cell Bank

JCRB1049 OVMIU

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