JCRB0094 P30/OHK
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0094 | Cell Name | P30/OHK |
|---|---|---|---|
| Profile | leukemia, acute non T non B lymphoblastic | Other Name | OHK |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 11 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | non T non B acute lymphoblastic leukemia (ALL) | Metastasis | |
| Tissue Metastasized | Genetics | CD5-,CD2-,Ia+,CD20-(revised to be negative at 2009/1/22),CD19+,J5+,TdT+,EBNA-,CD8+/- | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | PAS+,AcPase+,having pre B characteristic |
| Classify | tumor | Established by | Hirose,M. |
| Registered by | Minato,K. | Regulation for Distribution | Permission required. No commercial purpose. |
| Comment | CD8 antigen is induced by addition of TPA. Pre-preB ALL strain. | Year | 1986 |
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | Dilution every 4 days. |
| Cell Number on Passage | 10^5 cells/60mm dish | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:6983990 | A novel pre-T cell line derived from acute lymphoblastic leukemia. Hirose M,Minato K,Tobinai K,Ohira M,Ise T,Watanabe S,Shimoyama M,Taniwaki M,Abe T Gan. 1982 Aug;73(4):600-5 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0094 | Cell Name | P30/OHK |
|---|---|---|---|
| LOT No. | 111092 | Lot Specification | distribution |
| Medium | RPMI1640 medium (090892) with 10% fetal bovine serum (JRHB 1B1062) | Temperature | 37 C |
| Cell Density at Seeding | 1.7x10^5 cells/ml | Methods for Passages | suspension culture, dilution. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.2x10^6 |
| Viability at cell freezing (%) | 98.1 | Antibiotics Used | free |
| Passage Number | P14* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | M=44 | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0094 | Cell Name | P30/OHK |
|---|---|---|---|
| LOT No. | 011789 | Lot Specification | distribution |
| Medium | RPMI1640 + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | 10^5 cells/60mm dish | Methods for Passages | Dilution every 4 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 7.6x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P14* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | NT |
| Sterility: FUNGI | NT | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | D5S818:11,12 D13S317:10 D7S820:10,11 D16S539:9 VWA:14,21 TH01:9 AM:X TPOX:10,11 CSF1PO:9,11 |
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| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
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