JCRB0090 MB86

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0090 Cell Name   MB86
Profile   anti-Igh6-4 antibody-producing, IgM, (BALB/c mouse) Other Name   
Animal   mouse Strain   BALB/C
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   antibody-producing hybridoma
Case history    Metastasis   
Tissue Metastasized    Genetics   producing antibody to mouse IgM (b,e,z)
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   recognize Igh6-4
Classify   hybridoma Established by   Nishikawa,S.
Registered by   Nishikawa,S. Regulation for Distribution   free
Comment   This cell line produces monoclonal antibody recognizing IgM expressed by any B-cells of mouse. The cell line can be applied to a clonal analysis. Year   1986
Medium   RPMI1640 medium with 5 x 10^-5 M 2 mercapto ethanol and 10% fetal calf serum Methods for Passages   Dilution
Cell Number on Passage   split ratio=1/10 Race   
CO2 Conc.   5 % Tissue Sampling   NS1 x spleen
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J274
Reference
Pubmed id:2420716A monoclonal antibody against Igh6-4 determinant.
Nishikawa S,Sasaki Y,Kina T,Amagai T,Katsura Y
Immunogenetics. 1986;23(2):137-9




LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0090 Cell Name   MB86
LOT No.   072486 Lot Specification   distribution
Medium   RPMI1640 (5X10^-5 M 2ME) + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/10 Methods for Passages   Dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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