-Detailed Information [JCRB0086]-

JCRB0086 TALL-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0086	Cell Name	TALL-1
Profile	leukemia, acute T lymphoblastic	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	28
Identity	available	Tissue for Primary Cancer	hemo-lymphocytic
Case history	acute lymphoblastic leukemia	Metastasis
Tissue Metastasized		Genetics	T cell
Life Span	infinite	Crisis PDL
Morphology	lymphocyte-like	Character	T cell lymphoblastic leukemia. mode=95-101
Classify	tumor	Established by	Hiraki,S.
Registered by	Miyoshi,I.	Regulation for Distribution
Comment		Year	1986
Medium	RPMI1640 medium with 10% fetal calf serum	Methods for Passages	Dilution.
Cell Number on Passage	5x10^5 cells/ml	Race
CO2 Conc.	5 %	Tissue Sampling	bone marrow
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1736
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	909762

Reference
Pubmed id:2474678	Mutational analysis of the human immunodeficiency virus vpr open reading frame. Ogawa K,Shibata R,Kiyomasu T,Higuchi I,Kishida Y,Ishimoto A,Adachi A J Virol. 1989 Sep;63(9):4110-4
Pubmed id:305872	Establishment of a T-cell line from human lymphosarcoma. Hiraki S,Miyoshi I,Kubonishi I,Matsuda Y,Nakayama T,Kishimoto H,Machida K,Masuji H,Kimura I Gan. 1978 Feb;69(1):115-8
Pubmed id:197412	Human B cell, T cell and null cell leukaemic cell lines derived from acute lymphoblastic leukaemias. Miyoshi I,Hiraki S,Tsubota T,Kubonishi I,Matsuda Y,Nakayama T,Kishimoto H,Kimura I,Masuji H Nature. 1977 Jun 30;267(5614):843-4

Images

LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

08072008
062994
111787
071286
12172015
11112021
06282024

Cell No.	JCRB0086	Cell Name
LOT No.	08072008	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375)	Temperature	37 C
Cell Density at Seeding	4 x 10^4 cells/mL	Methods for Passages	dilution
Doubling Time	approx. 2 days	Cell Number in Vial (cells/1ml)	1.2 x 10^6
Viability at cell freezing (%)	85.7	Antibiotics Used	free
Passage Number	Unknown (6 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0086	Cell Name	TALL-1
LOT No.	062994	Lot Specification	distribution
Medium	RPMI1640 medium with 10% heat inactivated fetal bovine serum (JR Scientific,CO19407)	Temperature	37 C
Cell Density at Seeding	1.3x10^5 cells/ml	Methods for Passages	Simple dilution method.
Doubling Time	36.2 hr	Cell Number in Vial (cells/1ml)	1.02x10^7
Viability at cell freezing (%)	79.6	Antibiotics Used	free
Passage Number	P6*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB), LDH, NP
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:9,12 D13S317:8,9 D7S820:10,11 D16S539:9,10 VWA:17,19 TH01:7,9 AM:X,Y TPOX:8,11 CSF1PO:11,13
Adhesion	No	Exoteric Gene	NT
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0086	Cell Name	TALL-1
LOT No.	111787	Lot Specification	distribution
Medium	RPMI1640 + FCS 10%	Temperature	37
Cell Density at Seeding	5x10^5 cells/ml	Methods for Passages	Dilution.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.8x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P5*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD,LD,NP
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0086	Cell Name	TALL-1
LOT No.	071286	Lot Specification	distribution
Medium	RPMI1640 + FCS 10%	Temperature	37
Cell Density at Seeding	5x10^5 cells/ml	Methods for Passages	Dilution.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	9x10^5
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P6*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0086	Cell Name	TALL-1
LOT No.	12172015	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	2.1 - 3.0 x 105 cells/mL	Methods for Passages	Simple dilution (suspension culture).
Doubling Time	approx. 41 hrs	Cell Number in Vial (cells/1ml)	2.7 x 10^6
Viability at cell freezing (%)	82	Antibiotics Used	free
Passage Number	Unknown (6 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	No	Exoteric Gene	NT
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	82	Additional information

Cell No.	JCRB0086	Cell Name	TALL-1
LOT No.	11112021	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; BioWest Cat. # S1820-500)	Temperature	37 C
Cell Density at Seeding	3.4 - 4.8 x 10^4 cells/mL	Methods for Passages	Simple dilution (suspension culture).
Doubling Time	approx. 39 hrs.	Cell Number in Vial (cells/1ml)	2.9 x 10^6
Viability at cell freezing (%)	81.0	Antibiotics Used	free
Passage Number	Unknown (6 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	79.3	Additional information

Cell No.	JCRB0086	Cell Name	TALL-1
LOT No.	06282024	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K)	Temperature	37 C
Cell Density at Seeding	1 - 3 x 10^5 cells/mL	Methods for Passages	Simple dilution (suspension culture).
Doubling Time	approx. 45 hrs.	Cell Number in Vial (cells/1ml)	1.4 x 10^6
Viability at cell freezing (%)	91	Antibiotics Used	free
Passage Number	Unknown (8 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	88	Additional information