JCRB0080 LU99

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0080 Cell Name   LU99
Profile   giant cell carcinoma Other Name   LU99
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   63
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   giant cell carcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   lung, carcinoma
Classify   tumor Established by   Hirohashi,S. & Simosato,Y.
Registered by   Suzuki,K. Regulation for Distribution   free
Comment    Year   1986
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Mechanical dispersion.
Cell Number on Passage   split ratio=1/10 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3015
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907796
Reference
Pubmed id:3935627Giant cell carcinomas of the lung producing colony-stimulating factor in vitro and in vivo.
Yamada T,Hirohashi S,Shimosato Y,Kodama T,Hayashi S,Ogura T,Gamou S,Shimizu N
Jpn J Cancer Res. 1985 Oct;76(10):967-76

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0080 Cell Name   LU99
LOT No.   101896 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum (JRS DO10400). Temperature   37 C
Cell Density at Seeding   1-2x10^5 cells/ml Methods for Passages   Cells are recovered from dish by mechanical despersion.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.4x10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(type B), LDH, NP. (human)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:10,11
D7S820:11
D16S539:9
VWA:18
TH01:9
AM:X
TPOX:11
CSF1PO:10,15
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0080 Cell Name   LU99
LOT No.   061891 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum. Temperature   37 C
Cell Density at Seeding   dilution 1/10 Methods for Passages   Mechanical dispersion.
Doubling Time   21.1 hrs Cell Number in Vial (cells/1ml)   2.6x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0080 Cell Name   LU99
LOT No.   071687 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/10 Methods for Passages   Mechanical dispersion.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0080 Cell Name   LU99
LOT No.   10092014 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.0 - 2.5 x 10^5 cells/mL Methods for Passages   Mechanical dispersion and dilution. (LU99 is adherent cells but the attachment is weak, and can be detached by vigorous pipetting.)
Doubling Time   approx. 25 hrs Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:10,11
D7S820:11
D16S539:9
VWA:18
TH01:9
AM:X
TPOX:11
CSF1PO:10,15
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
Cell No.   JCRB0080 Cell Name   LU99
LOT No.   12052019 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 1.5 x 10^5 Methods for Passages   Mechanical dispersion and dilution. (LU99 is adherent cells but the attachment is weak, and can be detached by vigorous pipetting. Precooling of culture in PBS at 4C for 5 min before dispersion will facilitate the detachment of cells.)
Doubling Time   approx. 24 hrs. Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   91 Additional information   
Cell No.   JCRB0080 Cell Name   LU99
LOT No.   10052023 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437 Lot 1072322. Temperature   37 C
Cell Density at Seeding   2.5- 7.6 x 10^5 cells/mL Methods for Passages    Mechanical dispersion and dilution. (LU99 is adherent cells but the attachment is weak, and can be detached by vigorous pipetting. Precooling of culture in PBS at 4C for 5 min before dispersion will facilitate the detachment of cells.)
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   63.1 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:10,11
D7S820:11
D16S539:9
VWA:18
TH01:9
AM:X
TPOX:11
CSF1PO:10,15
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   69.7 Additional information   
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