JCRB0073 J-111

Cell information

Important Notice(s)

JCRB0073, IFO50005 J-111 is HeLa-derivative / H-111細胞のHeLa細胞汚染

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0073 Cell Name   J-111
Profile   The cell line deposited as a human acute monocytic leukemia but many data show this cell line is a subline of the HeLa. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   25
Identity   available Tissue for Primary Cancer   HeLa-contaminant
Case history   acute monocytic leukemia Metastasis   
Tissue Metastasized    Genetics   HeLa marker chromosome observed {371}. STR-PCR showed same pattern as HeLa cells.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Although an original description for this cell line is an acute monocytic leukemia, the cell line deposited to the cell bank anywhere in the world is a subline of the HeLa. See STR-PCR results.
Classify   tumor Established by   Osgood,E.E.
Registered by   Akiyama,M. Regulation for Distribution   
Comment   Hela markers observed. In.Vitro.14.469.1978. Please notice that this J-111 cell line is different from the CRL-8129 in ATCC although the name is the same. Year   1985
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum. Methods for Passages   Cells are harvested after treatment with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/3 Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 +
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2965
Reference
Pubmed id:566722Survey of ATCC stocks of human cell lines for HeLa contamination.
Lavappa KS
In Vitro. 1978 May;14(5):469-75
Pubmed id:14185313ANIMAL CELL STRAINS. THE CELL CULTURE COLLECTION COMMITTEE HAS ASSEMBLED AND CERTIFIED 23 STRAINS OF ANIMAL CELLS.
LEDLEY RS
Science. 1964 Oct 9;146(3641):241-3
Pubmed id:13260360Continuous tissue culture of leukocytes from human leukemic bloods by application of gradient principles.
OSGOOD EE,BROOKE JH
Blood. 1955 Oct;10(10):1010-22

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0073 Cell Name   J-111
LOT No.   030395 Lot Specification   FDSC/distribution
Medium   Eagle's minimal essential medium with non-essential AAs and 10% fetal bovine serum(BioCell 4001776) Temperature   37 C
Cell Density at Seeding   7.5x10^5 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.23x10^6
Viability at cell freezing (%)   100. Antibiotics Used   free
Passage Number   P7 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0073 Cell Name   J-111
LOT No.   102591 Lot Specification   distribution
Medium   MEM with non essential amino acids + FCS 10% Temperature   37 C
Cell Density at Seeding   1/3 dilut. Methods for Passages   0.02 % EDTA and 0.25 % trypsin treatment
Doubling Time   NT Cell Number in Vial (cells/1ml)   1x10^6/ml
Viability at cell freezing (%)   ND Antibiotics Used   free
Passage Number   P10 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0073 Cell Name   J-111
LOT No.   071889 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/3 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P8 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0073 Cell Name   J-111
LOT No.   092887 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/3 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P7 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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