-Detailed Information [JCRB0071]-

JCRB0071 BALL-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0071	Cell Name	BALL-1
Profile	Acute lymphoblastoid leukemia (ALL) cell line from human.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age
Identity	available	Tissue for Primary Cancer	hemo-lymphocytic
Case history	acute lymphoblastoid leukemia (ALL)	Metastasis
Tissue Metastasized		Genetics
Life Span	infinite	Crisis PDL
Morphology	lymphocyte-like	Character
Classify	tumor	Established by	Hiraki,S.
Registered by	Akiyama,M.	Regulation for Distribution	free
Comment		Year	1985
Medium	RPMI1640 medium with 10% fetal calf serum	Methods for Passages	Dilution, twice a week.
Cell Number on Passage	split ratio=1/3	Race
CO2 Conc.	5 %	Tissue Sampling	peripheral blood
Tissue Type	ALL

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1075

Reference
Pubmed id:2698048	Expression of cytokine genes in hematological malignancies. Okamura S,Hayashi S,Kawasaki C,Kondo S,Asano Y,Omori F,Otsuka T,Shibuya T,Harada M,Niho Y Nihon Ketsueki Gakkai Zasshi. 1989 Dec;52(8):1423-32
Pubmed id:197412	Human B cell, T cell and null cell leukaemic cell lines derived from acute lymphoblastic leukaemias. Miyoshi I,Hiraki S,Tsubota T,Kubonishi I,Matsuda Y,Nakayama T,Kishimoto H,Kimura I,Masuji H Nature. 1977 Jun 30;267(5614):843-4

Images

LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

09212012
053193
042190
100187
10182024

Cell No.	JCRB0071	Cell Name
LOT No.	09212012	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	3.4 x 10^5 cells/mL	Methods for Passages	Simple dilution. (suspension culture)
Doubling Time	approx. 28 hrs	Cell Number in Vial (cells/1ml)	1.1 x 10^6
Viability at cell freezing (%)	82.9	Antibiotics Used	free
Passage Number	Unknown (10 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0071	Cell Name	BALL-1
LOT No.	053193	Lot Specification	distribution
Medium	RPMI-1640 with 10% heat inactivated fetal bovine serum (Hyclone 115975)	Temperature	37 C
Cell Density at Seeding	3.5x10^5 cells/ml	Methods for Passages	Harvest cells by centrifuge and wash cells.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	5.75x10^4
Viability at cell freezing (%)	73.9	Antibiotics Used	free
Passage Number	P7	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	No	Exoteric Gene	NT
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0071	Cell Name	BALL-1
LOT No.	042190	Lot Specification	distribution
Medium	RPMI1640 + FCS 10%	Temperature	37
Cell Density at Seeding	dilution 1/3	Methods for Passages	Dilution, twice a week.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.2x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P5*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	NT
Sterility: FUNGI	NT	Isozyme Analysis	NT
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0071	Cell Name	BALL-1
LOT No.	100187	Lot Specification	distribution
Medium	RPMI1640 + FCS 10%	Temperature	37
Cell Density at Seeding	dilution 1/3	Methods for Passages	Dilution, twice a week.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	10^6
Viability at cell freezing (%)	92%	Antibiotics Used	free
Passage Number	P4*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD,LD,NP
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0071	Cell Name	BALL-1
LOT No.	10182024	Lot Specification	distribution
Medium	RPMI1640(GIBCO Cat #11875-093) with 10% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K)	Temperature	37 C
Cell Density at Seeding	2-2.82x10^5 cells/mL	Methods for Passages	dilution
Doubling Time	NT	Cell Number in Vial (cells/1ml)	4.08x10^6
Viability at cell freezing (%)	94	Antibiotics Used	free
Passage Number	p5*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:10,13 D13S317:9,12 D7S820:10,12 D16S539:9 VWA:14,18 TH01:7,9 AM:X TPOX:8,11 CSF1PO:10,12
Adhesion	No	Exoteric Gene	NT
Medium for Freezing	BAMBANKER (NIPPON Genetics Co., LTD, Cat.No.CS-02-001）	CO2 Conc.	5%
Viability immediately after thawing (%)	90	Additional information

Images