-Detailed Information [JCRB0066]-

JCRB0066 Mewo

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0066	Cell Name	Mewo
Profile	malignant melanoma	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	78
Identity	available	Tissue for Primary Cancer	melanoma, skin
Case history	malignant melanoma	Metastasis	Y
Tissue Metastasized	lymph node	Genetics
Life Span	infinite	Crisis PDL
Morphology	fibroblast-like	Character	Malignant melanoma (skin). Established from a melanotic lymph node metastasis. Slightly produces melanin.
Classify	tumor	Established by	Bean,M.A.
Registered by	Akiyama,M.	Regulation for Distribution
Comment		Year	1985
Medium	Eagle's minimum essential medium with non-essential amino acids and 10% fetal calf serum	Methods for Passages	Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage	split ratio=1/8	Race
CO2 Conc.	5 %	Tissue Sampling	lymph node
Tissue Type	malignant melanoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0445
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	908128

Reference
Pubmed id:2121721	Monoclonal antibodies to disialogangliosides: characterization of antibody-mediated cytotoxicity against human melanoma and neuroblastoma cells in vitro. Kawashima I,Tada N,Fujimori T,Tai T J Biochem. 1990 Jul;108(1):109-15
Pubmed id:2736210	Production of transforming growth factor-alpha in human tumour cell lines. Imanishi K,Yamaguchi K,Suzuki M,Honda S,Yanaihara N,Abe K Br J Cancer. 1989 May;59(5):761-5
Pubmed id:978138	Cell surface antigens of human malignant melanoma. II. Serological typing with immune adherence assays and definition of two new surface antigens. Shiku H,Takahashi T,Oettgen HF J Exp Med. 1976 Oct 1;144(4):873-81

Research results by users. Click！
Pubmed id:27595314	Stability and Bioavailability of Lentztrehaloses A, B, and C as Replacements for Trehalose. Wada S,Sawa R,Ohba S,Hayashi C,Umekita M,Shibuya Y,Iijima K,Iwanami F,Igarashi M J Agric Food Chem. 2016 Sep 28;64(38):7121-6
Pubmed id:2733038	Enhanced tumorigenicity, melanogenesis, and metastases of a human malignant melanoma after subdermal implantation in nude mice. Cornil I,Man S,Fernandez B,Kerbel RS J Natl Cancer Inst. 1989 Jun 21;81(12):938-44

Images

LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

04162002
052493
081391
112789
061587
01122015

Cell No.	JCRB0066	Cell Name
LOT No.	04162002	Lot Specification	distribution
Medium	Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum	Temperature	37 C
Cell Density at Seeding	6-9 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time	D.T. = ca 44 hrs	Cell Number in Vial (cells/1ml)	4.5 x 10^6
Viability at cell freezing (%)	95	Antibiotics Used	free
Passage Number	P27	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS-culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0066	Cell Name	Mewo
LOT No.	052493	Lot Specification	distribution
Medium	Eagle's minimal essential medium with Non-essential amino acids(GIBCO) and 10% FBS heat inactivated.	Temperature	37 C
Cell Density at Seeding	1:8 dilution	Methods for Passages	Harvest cells by 0.02% EDTA and 0.2% trypsin.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	3.2x10^6
Viability at cell freezing (%)	97.4	Antibiotics Used	free
Passage Number	P24	PDL	*
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion		Exoteric Gene	NT
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0066	Cell Name	Mewo
LOT No.	081391	Lot Specification	distribution
Medium	MEM w/ non essential amino acids + FCS 10%	Temperature	37 C
Cell Density at Seeding	dilution 1/8	Methods for Passages	0.02% EDTA and 0.25% trypsin.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.2x10^6
Viability at cell freezing (%)	95%	Antibiotics Used	free
Passage Number	P24	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0066	Cell Name	Mewo
LOT No.	112789	Lot Specification	distribution
Medium	MEM w/ non essential amino acids + FCS 10%	Temperature	37
Cell Density at Seeding	dilution 1/8	Methods for Passages	0.02% EDTA and 0.25% trypsin.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	5.7x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P24	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0066	Cell Name	Mewo
LOT No.	061587	Lot Specification	distribution
Medium	MEM w/ non essential amino acids + FCS 10%	Temperature	37
Cell Density at Seeding	dilution 1/8	Methods for Passages	0.02% EDTA and 0.25% trypsin.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	8x10^5
Viability at cell freezing (%)	92%	Antibiotics Used	free
Passage Number	P23	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD,LD,NP
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0066	Cell Name	Mewo
LOT No.	01122015	Lot Specification	distribution
Medium	Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	1.5 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time	approx. 31 hrs	Cell Number in Vial (cells/1ml)	1.5 x 10^6
Viability at cell freezing (%)	83	Antibiotics Used	free
Passage Number	P26	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)	83	Additional information