-Detailed Information [JCRB0024]-

JCRB0024 IM-9

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0024	Cell Name	IM-9
Profile	myeloma, multiple, B lymphoblastic	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age
Identity	available	Tissue for Primary Cancer	hemo-lymphocytic
Case history	female patient with multiple myeloma.	Metastasis
Tissue Metastasized		Genetics	B lymphoblasts synthesiz. IgGk(2.4mg/10^6 cells/d).
Life Span	infinite	Crisis PDL
Morphology	lymphocyte-like	Character	Immunoglobulin secreting human lymphoblast.
Classify	transformed	Established by	Buell,D.N.
Registered by	Suzuki,K.	Regulation for Distribution
Comment	The cell line has receptors for growth hormone and etc.	Year	1985
Medium	RPMI1640 medium with 10% fetal calf serum	Methods for Passages	Subculture twice a week by dilution.
Cell Number on Passage	10^5 cells/ml	Race
CO2 Conc.	5 %	Tissue Sampling	bone marrow
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	+	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1305

Reference
Pubmed id:4359334	Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture. Gavin JR 3rd,Roth J,Neville DM Jr,de Meyts P,Buell DN Proc Natl Acad Sci U S A. 1974 Jan;71(1):84-8

Images

LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

03302009
02262009
09172008
101293
100690
030689
041186
091098

Cell No.	JCRB0024	Cell Name
LOT No.	03302009	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, Lot 484375)	Temperature	37 C
Cell Density at Seeding	approx. 1 x 10^5 cells/ml	Methods for Passages	Simple dilution, passage twice a week.
Doubling Time	approx. 23 hrs	Cell Number in Vial (cells/1ml)	5.8 x 106
Viability at cell freezing (%)	88.3	Antibiotics Used	free
Passage Number	Unknown (4 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0024	Cell Name	IM-9
LOT No.	02262009	Lot Specification	distribution
Medium	RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051)	Temperature	37 C
Cell Density at Seeding	0.18-1.2x10^5 cells/sq.cm	Methods for Passages	dilution
Doubling Time	NT	Cell Number in Vial (cells/1ml)	4.94x10^6
Viability at cell freezing (%)	97.9	Antibiotics Used	free
Passage Number	p4*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:13 D13S317:9,11 D7S820:11,12 D16S539:9,13 VWA:14,17 TH01:6,9.3 AM:X TPOX:11 CSF1PO:10,11
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Cell Banker BLC-1(Nihon Zenyaku Industries)	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0024	Cell Name
LOT No.	09172008	Lot Specification	distribution
Medium	RPM 1640 medium with 10% fetal bovine serum	Temperature	37 C
Cell Density at Seeding	3 - 6 x 10^5 cells/ml	Methods for Passages	Simple dilution
Doubling Time	approx. 1 day	Cell Number in Vial (cells/1ml)	6.3 x 10^6
Viability at cell freezing (%)	82.4	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0024	Cell Name	IM-9
LOT No.	101293	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (JRS, DO10400)	Temperature	37 C
Cell Density at Seeding	1x10^5 cells/ml	Methods for Passages	Simply dilute to fresh medium at the concentration above.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.74x10^6
Viability at cell freezing (%)	71.5	Antibiotics Used	free
Passage Number	P6* (P42 on ampule)	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	NT
Sterility: FUNGI	NT	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	No	Exoteric Gene	from lot.052785 by Ohnishi,K.
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0024	Cell Name	IM-9
LOT No.	100690	Lot Specification	distribution
Medium	RPMI1640 + FCS 10%	Temperature	37
Cell Density at Seeding	10^5 cells/ml	Methods for Passages	Subculture twice a week by dilution.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	3.5x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P3*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	NT
Sterility: FUNGI	NT	Isozyme Analysis	NT
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)	D5S818:13 D13S317:9,11 D7S820:11,12 D16S539:9,13 VWA:14,17 TH01:6,10 AM:X TPOX:11 CSF1PO:10,11
Adhesion		Exoteric Gene	NT
Medium for Freezing		CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0024	Cell Name	IM-9
LOT No.	030689	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal calf serum	Temperature	37
Cell Density at Seeding	10^5 cells/ml	Methods for Passages	Subculture twice a week by dilution.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	4.9x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P3*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	Culture medium with 5% DMSO.	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0024	Cell Name	IM-9
LOT No.	041186	Lot Specification	distribution
Medium	RPMI1640 + FCS 10%	Temperature	37
Cell Density at Seeding	10^5 cells/ml	Methods for Passages	Subculture twice a week by dilution.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.2x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P3*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0024	Cell Name
LOT No.	091098	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121)	Temperature	37 C
Cell Density at Seeding	1-2 x 10^5	Methods for Passages	Dilution
Doubling Time		Cell Number in Vial (cells/1ml)	4.2 x 10^6
Viability at cell freezing (%)	91.0	Antibiotics Used	free
Passage Number	P5*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information