JCRB1024 Kasumi-6

Cell information

Important Notice(s)

On the particles appear in cultures of Kasumi-series cell lines

Procedure for addition of GM-CSF

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1024 Cell Name   Kasumi-6
Profile   Human acute myeloid leukemia cell line with dominant negative mutation in the C/EBP alpha gene. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   64
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute myeloid leukemia, subtype M2(AML FAB M2). Relapsed AML. Metastasis   
Tissue Metastasized    Genetics   45,XY,-9,add(12)(p11),add(13)(p11) (20 metaphase cells scored). CG transitional mutation at 1063 of the C/EBP alpha gene producing P42 and P30 isoform endogenously.
Life Span   infinite Crisis PDL   
Morphology   myeloblast Character   Mononuclear cells separated by ficoll conray gradient.
Classify   tumor Established by   Asou,H. et.al.
Registered by   Asou,H. Regulation for Distribution   
Comment   Cell culture started on January 6, 1999. Year   2002
Medium   RPMI-1640 medium with GM-CSF (original description was 2 ng/mL but currently 10 ng/mL is used in JCRB Cell Bank) and 20% fetal bovine serum at 37 C in 5% CO2. Methods for Passages   dilution
Cell Number on Passage   1.0 x 10^6 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0614
Reference
Pubmed id:12508245Establishment of the acute myeloid leukemia cell line Kasumi-6 from a patient with a dominant-negative mutation in the DNA-binding region of the C/EBPalpha gene.
Asou H,Gombart AF,Takeuchi S,Tanaka H,Tanioka M,Matsui H,Kimura A,Inaba T,Koeffler HP
Genes Chromosomes Cancer. 2003 Feb;36(2):167-74


Research results by users. Click！
Pubmed id:32180206 Kasumi leukemia cell lines: characterization of tumor genomes with ethnic origin and scales of genomic alterations.
Kasai F,Asou H,Ozawa M,Kobayashi K,Kuramitsu H,Satoh M,Kohara A,Kaneko Y,Kawamura M
Hum Cell. 2020 Jul;33(3):868-876

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1024 Cell Name   Kasumi-6
LOT No.   11222013 Lot Specification   distribution2
Medium   RPMI1640 medium(GIBCO) with 10ng/ml GM-CSF(catalog No.11875) and 20% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.8-5.0x10^5 cells/ml Methods for Passages   Dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.6x10^6
Viability at cell freezing (%)   76.1 Antibiotics Used   free
Passage Number   p14* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:8,12
D7S820:9,11
D16S539:9,10
VWA:17
TH01:6,9
AM:X,Y
TPOX:8,9
CSF1PO:10,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1024 Cell Name   Kasumi-6
LOT No.   10282013 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10ng/ml GM-CSF(catalog No.11875) and 20% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37C
Cell Density at Seeding   1.8-5.0x10^5 cells/ml Methods for Passages   Dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.7x10^6
Viability at cell freezing (%)   85.0 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:8,12
D7S820:9,11
D16S539:9,10
VWA:17
TH01:6,9
AM:X,Y
TPOX:8,9
CSF1PO:10,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5%
Viability immediately after thawing (%)   83.4% Additional information   
Images
Cell No.   JCRB1024 Cell Name   Kasumi-6
LOT No.   11082002 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1024 Cell Name   Kasumi-6
LOT No.   04052017 Lot Specification   distribution
Medium   RPMI1640(GIBCO) medium with 10ng/ml GM-CSF(GIBCO) and 20% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   2.00-5.0x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.86x10^6
Viability at cell freezing (%)   92.46 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:8,12
D7S820:9,11
D16S539:9,10
VWA:17
TH01:6,9
AM:X,Y
TPOX:8,9
CSF1PO:10,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   We recommend to start cell density more than 1.5x10^6 cells/ml and change medium every four days.
Images
Cell No.   JCRB1024 Cell Name   Kasumi-6
LOT No.   07022018 Lot Specification   distribution
Medium   RPMI1640(GIBCO) medium with 10ng/ml GM-CSF(GIBCO) and 20% heat inactivated fetal bovine serum(SIGMA Cat#172012, Lot 12J396) Temperature   37 C
Cell Density at Seeding   2.0-5.0x10^5 cells/ml Methods for Passages   Suspension culture
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.6x10^6
Viability at cell freezing (%)   88.5 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:8,12
D7S820:9,11
D16S539:9,10
VWA:17
TH01:6,9
AM:X,Y
TPOX:8,9
CSF1PO:10,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Images
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