JCRB1016 HSC-5
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1016 | Cell Name | HSC-5 |
|---|---|---|---|
| Profile | A cell line derived from human skin squamous cell carcinoma having anaplastic epithelioid features. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 75 |
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | ulcerated tumor on the upper part of the right auricle. | Metastasis | |
| Tissue Metastasized | Genetics | Modal number of chromosome is reported to be 76. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | The cells lost their ability to defferentiate to keratinize. The cells express cytokeratins 5, 8, 13, 18 and 19 {5064}. |
| Classify | tumor | Established by | Hozumi,Y. et.al. |
| Registered by | Hozumi,Y. | Regulation for Distribution | |
| Comment | Year | 2001 | |
| Medium | Iscove's modified Dulbecco's medium containing 10% fetal bovine serum. | Methods for Passages | Cells harvested after treatment with 0.02% EDTA and 0.05% trypsin. Subculture once a week. |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5 % | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1693926 | Human squamous cell carcinoma from skin: establishment and characterization of a new cell line (HSC-5). Hozumi Y,Kondo S,Shimoura T,Aso K J Dermatol. 1990 Mar;17(3):143-8 |
| Pubmed id:6169443 | A new small (40 kd) keratin filament protein made by some cultured human squamous cell carcinomas. Wu YJ,Rheinwald JG Cell. 1981 Sep;25(3):627-35 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1016 | Cell Name | HSC-5 |
|---|---|---|---|
| LOT No. | 08062007 | Lot Specification | distribution |
| Medium | Iscove's modified Dulbecco's medium with 10% fetal bovine serum (lot:GIBCO484375). | Temperature | 37C |
| Cell Density at Seeding | 1.2 x 10^5cells/ml (twice/week) | Methods for Passages | 0.25% trypsin and 0.02% EDTA in PBS |
| Doubling Time | approx. 1 day | Cell Number in Vial (cells/1ml) | 6.0 x 10^6 cells |
| Viability at cell freezing (%) | 85.9% | Antibiotics Used | free |
| Passage Number | P131 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B) AST |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:11 D13S317:9 D7S820:8,11 D16S539:9,12 VWA:16,17 TH01:6,7 AM:X,Y TPOX:11 CSF1PO:10,11 |
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| Adhesion | Yea | Exoteric Gene | |
| Medium for Freezing | 70% Culture medium + 20% FCS + 10% DMSO | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1016 | Cell Name | HSC-5 |
|---|---|---|---|
| LOT No. | 05232018 | Lot Specification | distribution |
| Medium | Iscove's modified Dulbecco's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 0.8 - 2.2 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 22 hrs. | Cell Number in Vial (cells/1ml) | 1.5 x 10^6 |
| Viability at cell freezing (%) | 96.1 | Antibiotics Used | free |
| Passage Number | P131 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - IMDM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 96.1 | Additional information |