JCRB1008 HFb16d

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1008 Cell Name   HFb16d
Profile   Normal human fibroblast cell line derived from foreskin which was transfected by the HPV16 DNA. No teromerase activity. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   
Identity   available Tissue for Primary Cancer   skin
Case history   normal Metastasis   
Tissue Metastasized    Genetics   Cells transfected by HPV16 DNA. No telomerase activity. Cells can be passaged over 25 generations at 1/5 split ratio.
Life Span   not defined Crisis PDL   unknown
Morphology   fibroblast-like Character   Normal fibroblast human cells with HPV16 DNA.
Classify   transformed Established by   Yasumoto,S.
Registered by   Yasumoto,S. Regulation for Distribution   not applied.
Comment   Cells line is unique shown by the STR-PCR method. Cells registered as JCRB on 04/15/2002. Year   2000
Medium   Dulbecco's modified Eagle's medium with 10 % fetal bovine serum. Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.01% EDTA.
Cell Number on Passage   not defined Race   
CO2 Conc.   5 % Tissue Sampling   foreskin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2941
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1008 Cell Name   HFb16d
LOT No.   12152003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO Cat. # 10091, Lot 3762782S) Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca. 3 days Cell Number in Vial (cells/1ml)   7.9 x 10^5
Viability at cell freezing (%)   95.5 Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1008 Cell Name   HFb16d
LOT No.   04042025 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   3 - 8 x 10^3 cells/cm2 Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   5.5 x 10^5
Viability at cell freezing (%)   84.8 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   91.7 Additional information   
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