JCRB1005 NCC-IT-A3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1005 Cell Name   NCC-IT-A3
Profile   Human teratocarcinoma cell line from anterior mediastinum. Other Name   NCCIT
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   24
Identity   available Tissue for Primary Cancer   teratocarcinoma
Case history   teratocarcinoma Metastasis   
Tissue Metastasized    Genetics   Cells are expressing HST-1 oncogene
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   teratocarcinoma from anterior mediastinum
Classify   tumor Established by   Teshima, S.
Registered by   Teshima, S. Regulation for Distribution   
Comment   Cells were obtained from tissue and were cloned later by the dilution method. Year   1990
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Mechanical pipetting is enough, which was informed originally.
Cell Number on Passage   split ratio=1/5 - 1/10 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   anterior mediastinum
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3045
Reference
Pubmed id:3060118Differential expression of two homologous and clustered oncogenes, Hst1 and Int-2, during differentiation of F9 cells.
Yoshida T,Muramatsu H,Muramatsu T,Sakamoto H,Katoh O,Sugimura T,Terada M
Biochem Biophys Res Commun. 1988 Dec 15;157(2):618-25
Pubmed id:2974287Two homologous oncogenes, HST1 and INT2, are closely located in human genome.
Wada A,Sakamoto H,Katoh O,Yoshida T,Yokota J,Little PF,Sugimura T,Terada M
Biochem Biophys Res Commun. 1988 Dec 15;157(2):828-35
Pubmed id:3178812Expression of the HST1 oncogene in human germ cell tumors.
Yoshida T,Tsutsumi M,Sakamoto H,Miyagawa K,Teshima S,Sugimura T,Terada M
Biochem Biophys Res Commun. 1988 Sep 30;155(3):1324-9
Pubmed id:2895649Cloned hst gene from normal human leukocyte DNA transforms NIH3T3 cells.
Sakamoto H,Yoshida T,Nakakuki M,Odagiri H,Miyagawa K,Sugimura T,Terada M
Biochem Biophys Res Commun. 1988 Mar 30;151(3):965-72

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1005 Cell Name   
LOT No.   01192004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS Lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   6 - 7 x 10^4 cells/ml Methods for Passages   Pipetting, or 0.25% tryspin and 0.02% EDTA. The former was recommended by depositor, but apparently the latter method resulted good recovery.
Doubling Time   27 hrs (max) Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   91.7 Antibiotics Used   free
Passage Number   P15* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1005 Cell Name   NCC-IT-A3
LOT No.   07192002 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   1.2 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested by piptting. Cells are quite weak when vigorous handling is done when subculture.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   82.5 Antibiotics Used   free
Passage Number   P14* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human, not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:11
D7S820:10
D16S539:9,12
VWA:14,18
TH01:7,9
AM:X
TPOX:8
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture mediium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1005 Cell Name   NCC-IT-A3
LOT No.   11162017 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   92.1 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   92.1 Additional information   
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