JCRB0538 TIG-109
Cell information
Important Notice(s)Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0538 | Cell Name | TIG-109 |
|---|---|---|---|
| Profile | Normal diploid human skin cells. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 39 |
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | normal fibroblast, 2n=46, XX. | |
| Life Span | finite | Crisis PDL | PD45 |
| Morphology | fibroblast-like | Character | normal diploid human skin |
| Classify | normal | Established by | TIG |
| Registered by | TIG | Regulation for Distribution | |
| Comment | Year | 2002 | |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum. Cultured at 37 C. | Methods for Passages | |
| Cell Number on Passage | Split ratio = 1/4. | Race | Japanese |
| CO2 Conc. | Tissue Sampling | skin | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Images |
|---|
 |
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0538 | Cell Name | |
|---|---|---|---|
| LOT No. | 02122010 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099). This lot was frozen using DMSO. | Temperature | 37 C |
| Cell Density at Seeding | approx. 3 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA. Split ratio = 1:6 |
| Doubling Time | approx. 42 hrs | Cell Number in Vial (cells/1ml) | 8.1 x 105 |
| Viability at cell freezing (%) | 97.5 | Antibiotics Used | free |
| Passage Number | Unknown | PDL | 21 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0538 | Cell Name | TIG-109 |
|---|---|---|---|
| LOT No. | 02062024 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) | Temperature | 37 C |
| Cell Density at Seeding | approx. 3 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 2 days | Cell Number in Vial (cells/1ml) | 5.5 x 10^5 |
| Viability at cell freezing (%) | 97 | Antibiotics Used | free |
| Passage Number | Unknown (3 at bank) | PDL | 20.3 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:11,12 D13S317:8 D7S820:12,13 D16S539:9,11 VWA:16,18 TH01:7,9 AM:X TPOX:8 CSF1PO:9,10 |
|
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 92 | Additional information |