JCRB0537 TIG-108
Cell information
Important Notice(s)Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0537 | Cell Name | TIG-108 |
|---|---|---|---|
| Profile | skin, normal diploid fibroblast | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 40 |
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | normal diploid, 2n = 46, XX. | |
| Life Span | finite | Crisis PDL | PDL42 |
| Morphology | fibroblast-like | Character | normal fibroblast |
| Classify | normal | Established by | TIG |
| Registered by | TIG | Regulation for Distribution | |
| Comment | Year | 2002 | |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum. | Methods for Passages | |
| Cell Number on Passage | Split ratio = 1/4. | Race | Japanese |
| CO2 Conc. | Tissue Sampling | skin | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | tested | ||||||
| HCV | - | HTLV-1 | - | ||||||
| HIV-1 | - | HTLV-2 | - | ||||||
| HIV-2 | - | HAV | - | ||||||
|
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| Notes | |||||||||
| Images |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0537 | Cell Name | |
|---|---|---|---|
| LOT No. | 10122010 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099). This lot was frozen using DMSO. | Temperature | 37C |
| Cell Density at Seeding | 2.2 - 3.4 x 10^4 cells/ml | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 51 hrs. | Cell Number in Vial (cells/1ml) | 7.4 x 10^5 |
| Viability at cell freezing (%) | 94.9 | Antibiotics Used | free |
| Passage Number | Unknown (4 at bank) | PDL | 21 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0537 | Cell Name | TIG-108 |
|---|---|---|---|
| LOT No. | 10262015 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). This lot was frozen using DMSO. | Temperature | 37 C |
| Cell Density at Seeding | 2.0 - 5.4 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 31 hrs | Cell Number in Vial (cells/1ml) | 1.0 x 10^6 |
| Viability at cell freezing (%) | 90 | Antibiotics Used | free |
| Passage Number | Unknown (3 at bank) | PDL | 22 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 90 | Additional information |