JCRB0200 KMLS-1

Cell information

Important Notice(s)

KMLS-1: cultivation is relatively difficult.

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0200 Cell Name   KMLS-1
Profile   liposarcoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   retroperitoneum
Case history   liposarcoma arose at retroperitoneum Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   tumor Established by   Shuin,T.
Registered by   Shuin,T. Regulation for Distribution   
Comment    Year   2002
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum Methods for Passages   0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   split 1:2 - 1:3 Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2980
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0200 Cell Name   
LOT No.   04142008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS Lot; GIBCO Cat. No. 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   1.0 - 2.3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   88 - 175 hrs Cell Number in Vial (cells/1ml)   3.5 x 10^5
Viability at cell freezing (%)   78.2 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0200 Cell Name   KMLS-1
LOT No.   03242015 Lot Specification   distribution
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   3.12-8.02x10^3cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.27x10^6
Viability at cell freezing (%)   91.8 Antibiotics Used   free
Passage Number   p23 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:11,12
D7S820:11,13
D16S539:9,10
VWA:17,18
TH01:6,7
AM:X
TPOX:9,11
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB0200 Cell Name   KMLS-1
LOT No.   09112019 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.0 - 3.6 x 10^6 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   Very slow (approx. 8 days). Cell Number in Vial (cells/1ml)   2.0 x 10^5
Viability at cell freezing (%)   69.4 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   71.3 Additional information   
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