JCRB0199 huH-1

Cell information

Important Notice(s)

本細胞株の分譲に際しての寄託者の承諾について (On the distribution of this cell line. An approval of depositor is required.)

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0199 Cell Name   huH-1
Profile   Human hepatoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   53
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   hepatoma, HBs-antigen carrier Metastasis   No
Tissue Metastasized    Genetics   Modal chromosome number is 69.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   The huH-1 cell line produce tumors in nude mice. Cells are producing HBs-antigen continuously in culture medium. Tyrosine aminotransferase activity was induced by dexamethasone as well as by butyryl c-AMP.
Classify   tumor Established by   Hoh,H.
Registered by   Hoh,H. Regulation for Distribution   Depositor's approval is required.
Comment    Year   2002
Medium   DM-160 medium with 10% fetal bovine serum (original descripton). Current medium for propagation is DMEM + 10% FBS. Methods for Passages   
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV + HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes HB virus genome fragment was detected by realtime PCR. However, viral particles and HBe antigen (core protein of HBV) were not detected (Gan. 1981,72(1):178-9). Also, there was no detectable free viral linear DNA at 3.2 kb or free viral closed circular DNA in the 2.3 kb region by Southern blotting for DNAs extracted from huH-1 (Nucleic Acids Res. 1983 Aug 25;11(16):5391-402). Therefore infectious HBV particles will not be produced by huH-1.

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2956
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1298146
Reference
Pubmed id:6310497Rearrangement of the surface antigen gene of hepatitis B virus integrated in the human hepatoma cell lines.
Koike K,Kobayashi M,Mizusawa H,Yoshida E,Yaginuma K,Taira M
Nucleic Acids Res. 1983 Aug 25;11(16):5391-402
Pubmed id:6286115Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.
Nakabayashi H,Taketa K,Miyano K,Yamane T,Sato J
Cancer Res. 1982 Sep;42(9):3858-63
Pubmed id:6287253Metabolic activation of benzo[a]pyrene, aflatoxin B1, and dimethylnitrosamine by a human hepatoma cell line.
Huh N,Nemoto N,Utakoji T
Mutat Res. 1982 Jun;94(2):339-48
Pubmed id:6268479Production of HBs-antigen by two new human hepatoma cell lines and its enhancement by dexamethasone.
Huh N,Utakoji T
Gan. 1981 Feb;72(1):178-9

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0199 Cell Name   
LOT No.   07192012 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   0.9 - 1.4 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 31 hrs Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   94.7 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0199 Cell Name   
LOT No.   02172003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   1:4 - 1:6 split Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca. 36 hrs Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0199 Cell Name   huH-1
LOT No.   08102017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.8 - 2.1 x 10^5 Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 39 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   92.4 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   92.4 Additional information   
Cell No.   JCRB0199 Cell Name   huH-1
LOT No.   04022021 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.7 - 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 35 hrs. Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
Cell No.   JCRB0199 Cell Name   huH-1
LOT No.   01292025 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (GIBCO:catalog No.11885-084) with 10% fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K). Temperature   37 C
Cell Density at Seeding   1.5-3.3x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:10,12
D7S820:11
D16S539:9
VWA:17,18
TH01:6,9
AM:X,Y
TPOX:8,11
CSF1PO:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
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