JCRB0198 OSC-19

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0198 Cell Name   OSC-19
Profile   Human squamous cell carcinoma cell line from tongue tumor with serum free cell culture. Other Name   OSC19
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   61
Identity   available Tissue for Primary Cancer   oral
Case history   squamous cell carcinoma of tongue. Metastasis   Yes
Tissue Metastasized   Neck lymph node. Genetics   EGF receptor.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   EGF (1-100ng/ml) inhibited the cell growth. The cell could not grow in soft agar but the EGF stimulated colony formation in the soft agar.
Classify   tumor Established by   Yokoi,T.
Registered by   Yokoi,T. Regulation for Distribution   
Comment    Year   1990
Medium   1 to 1 mixture of Dulbecco's modified Eagle's medium with F-12 medium. Methods for Passages   Cells were washed three times with Ca-Mg free Hank's balanced salt solution (HBSS), then treated with 0.01% trypsin and 0.01% EDTA in HBSS.
Cell Number on Passage   1 x 10^5 cells in 60mm dish. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   tongue
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3086
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1298362
Reference
Pubmed id:8514607The expression of invasive behavior of differentiated squamous carcinoma cell line evaluated by an in vitro invasion model.
Kawahara E,Okada Y,Nakanishi I,Iwata K,Kojima S,Kumagai S,Yamamoto E
Jpn J Cancer Res. 1993 Apr;84(4):409-18

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0198 Cell Name   OSC-19
LOT No.   04012012 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, serum-free. (This cell line can also be cultivated with adding serum.) Temperature   37 C
Cell Density at Seeding   2.0 - 3.2 x 10^4 cells/mL Methods for Passages   0.01% trypsin and 0.01% EDTA, trypsin activity was terminated by soybean trypsin inhibitor (serum-free culture)
Doubling Time   approx. 50 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   94.1 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0198 Cell Name   
LOT No.   04132010 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, serum-free. (This cell line can also be cultivate with adding serum.) Temperature   37 C
Cell Density at Seeding   1.8 - 3.3 x 10^4 cells/ml Methods for Passages   0.01% trypsin and 0.01% EDTA, trypsin activity was terminated by soybean trypsin inhibitor (serum-free culture)
Doubling Time   60 hrs Cell Number in Vial (cells/1ml)   8.9 x 10^5
Viability at cell freezing (%)   92.5 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0198 Cell Name   
LOT No.   04102007 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, serum-free. (This cell line can also be cultivate with adding serum.) Temperature   37 C
Cell Density at Seeding   4-6 x 10^4 cells/ml Methods for Passages   0.01% trypsin and 0.01% EDTA, trypsin activity was terminated by soybean trypsin inhibitor (serum-free culture)
Doubling Time   >3 days (serum-free) Cell Number in Vial (cells/1ml)   6.2 x 10^5
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP and G6PD, LD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0198 Cell Name   OSC-19
LOT No.   06292016 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, serum-free. (This cell line can also be cultivated with adding serum.) Temperature   37 C
Cell Density at Seeding   1.0 - 2.4 x 10^4 cells/mL Methods for Passages   0.01% trypsin and 0.01% EDTA, trypsin activity was terminated by soybean trypsin inhibitor (serum-free culture)
Doubling Time   2 - 6 days (serum-free) Cell Number in Vial (cells/1ml)   6.9 x 10^5
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose in culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   93 Additional information   
Cell No.   JCRB0198 Cell Name   OSC-19
LOT No.   05112018 Lot Specification   distribution
Medium    1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, serum-free. (This cell line can also be cultivated with adding serum.) Temperature   37 C
Cell Density at Seeding   1.9 -3.0 x 10^4 cells/mL Methods for Passages   0.01% trypsin and 0.01% EDTA, trypsin activity was terminated by soybean trypsin inhibitor (serum-free culture)
Doubling Time   approx. 61 hrs. Cell Number in Vial (cells/1ml)   7.9 x 10^5
Viability at cell freezing (%)   77.5 Antibiotics Used   free
Passage Number   Unknown (12 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   77.5 Additional information   
Cell No.   JCRB0198 Cell Name   OSC-19
LOT No.   02272025 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, serum-free. (This cell line can also be cultivated with adding serum.) Temperature   37 C
Cell Density at Seeding   0.3 - 1.7 x 10^5 cells/mL Methods for Passages   0.01% trypsin and 0.01% EDTA, trypsin activity was terminated by soybean trypsin inhibitor (serum-free culture)
Doubling Time   approx. 68 hrs. Cell Number in Vial (cells/1ml)   8.7 x 10^5
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   Unknown (12 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,13
D13S317:12
D7S820:9,11
D16S539:9,12
VWA:14,18
TH01:9
AM:X,Y
TPOX:11
CSF1PO:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS (Nichirei Cat. # 174112, Lot 20M00K) - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   83 Additional information   
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