JCRB0001 F9-41

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0001 Cell Name   F9-41
Profile   Mouse testicular teratocarcinoma cell line. Other Name   F9
Animal   mouse Strain   129
Genus   Mus Species   musculus
Sex   M Age   
Identity   not done Tissue for Primary Cancer   teratoma
Case history   testicular teratocarcinoma Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   cells are differentiated by retinoic acit or cAMP
Classify   transformed Established by   Bernstine et al.
Registered by   Matsumura,T. Regulation for Distribution   Not regulated
Comment   Maximum cell density=1-2x10^7/10D. Do not culture more than 1 month. Year   1985
Medium   Dulbecco's modified Eagle's medium with 15% fetal calf serum. Methods for Passages   Harvest cells after treatment with 0.01% EDTA and 0.125% trypsin. Subculture every two days and disperse cells completely before scoring cell number.
Cell Number on Passage   1.5 x 10^6 cells/100mm dish Race   NT
CO2 Conc.   5 % Tissue Sampling   testis
Tissue Type   teratocarcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8300
Reference
Pubmed id:1706663Characterization of a murine beta 1-4 galactosyltransferase expressed in COS-1 cells.
Nakazawa K,Furukawa K,Kobata A,Narimatsu H
Eur J Biochem. 1991 Mar 14;196(2):363-8
Pubmed id:6164928Cell interactions modulate embryonal carcinoma cell differentiation into parietal or visceral endoderm.
Hogan BL,Taylor A,Adamson E
Nature. 1981 May 21;291(5812):235-7
Pubmed id:6250719Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP.
Strickland S,Smith KK,Marotti KR
Cell. 1980 Sep;21(2):347-55
Pubmed id:214238The induction of differentiation in teratocarcinoma stem cells by retinoic acid.
Strickland S,Mahdavi V
Cell. 1978 Oct;15(2):393-403
Pubmed id:1103673[Mouse teratocarcinoma: differentiation in cultures of a multipotential primitive cell line (author's transl)].
Nicolas JF,Dubois P,Jakob H,Gaillard J,Jacob F
Ann Microbiol (Paris). 1975 Jan;126(1):3-22
Pubmed id:4521215Alkaline phosphatase activity in mouse teratoma.
Berstine EG,Hooper ML,Grandchamp S,Ephrussi B
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3899-903

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0001 Cell Name   F9-41
LOT No.   022294 Lot Specification   distribution
Medium   Dalbecco's modified Eagle's medium with 15% fetal bovine serum (J.R.S. lot.DO10400) Temperature   37 C
Cell Density at Seeding   1.5x10^5 cells/ml Methods for Passages   Wash cells with PBS(-) and treated with 0.125%-trypsin and 0.01% EDTA for 5-10 minutes.
Doubling Time   22.6Hrs. Cell Number in Vial (cells/1ml)   1.15x10^7
Viability at cell freezing (%)   87.6 Antibiotics Used   free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD, NP (G6PD not detected)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0001 Cell Name   F9-41
LOT No.   041690 Lot Specification   distribution
Medium   Dulbecco`s modified Eagle's medium with 15% fetal calf serum (10% can be applicable). Temperature   37 C
Cell Density at Seeding   1.5X10^6/10D Methods for Passages   0.01% EDTA and 0.125% trypsin. Subculture every two days and disperse cells completely.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0001 Cell Name   F9-41
LOT No.   051288 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 15% fetal calf serum Temperature   37 C
Cell Density at Seeding   1.5X10^5 cells/ml Methods for Passages   0.01% EDTA and 0.125% trypsin. Subculture every two days and disperse cells completely.
Doubling Time   D.T.=10.27hr Cell Number in Vial (cells/1ml)   8.2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,NP,G6PD
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0001 Cell Name   F9-41
LOT No.   021286 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 15% fetal calf serum Temperature   37 C
Cell Density at Seeding   1.5X10^6 cells/100mm dish Methods for Passages   0.01% EDTA and 0.125% trypsin. Subculture every two days and disperse cells completely.
Doubling Time   10-12 hr Cell Number in Vial (cells/1ml)   3x10^6
Viability at cell freezing (%)   64% Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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