JCRB0190 KYSE70
Cell information
Important Notice(s)Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0190 | Cell Name | KYSE70 |
|---|---|---|---|
| Profile | Poorly differentiated human squamous cell carcinoma from esophageal cancer. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 77 |
| Identity | available | Tissue for Primary Cancer | esophagus |
| Case history | esophageal cancer, middle intrathoracic esophagus, poorly differentiated squamous cell carcinoma. | Metastasis | No |
| Tissue Metastasized | Genetics | Mode of chromosome number = 60-70. DNA fingerprint indicated the cell line was unique. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Cell line was established from inplanted tumor of nude mice (only initial passage). |
| Classify | Established by | Shimada,Y. et.al. | |
| Registered by | Shimada,Y. | Regulation for Distribution | |
| Comment | Year | 2001 | |
| Medium | Ham's F12/RPMI1640 medium containing 20% fetal calf serum for primary culture. | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5 % | Tissue Sampling | esophagus |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:9033652 | Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines. Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K Int J Cancer. 1997 Feb 7;70(4):437-42 |
| Pubmed id:8575860 | Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors. Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K Int J Cancer. 1996 Jan 26;65(3):372-6 |
| Pubmed id:1728357 | Characterization of 21 newly established esophageal cancer cell lines. Shimada Y,Imamura M,Wagata T,Yamaguchi N,Tobe T Cancer. 1992 Jan 15;69(2):277-84 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0190 | Cell Name | KYSE70 |
|---|---|---|---|
| LOT No. | 06102005 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 2% fetal bovine serum (FBS lot; GIBCO 4218333S) | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | 0.25% trypsin and 0.02% EDTA (original description in paper was 200 units/ml trypsin and 0.01% EDTA in Ca, Mg-free PBS) | |
| Doubling Time | approx. 25 hrs | Cell Number in Vial (cells/1ml) | 5.7 x 10^6 |
| Viability at cell freezing (%) | 97.6 | Antibiotics Used | free |
| Passage Number | Unknown (5 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:12 D13S317:11,12 D7S820:8,11 D16S539:13 VWA:16,18 TH01:7 AM:X TPOX:8 CSF1PO:12 |
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| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0190 | Cell Name | KYSE70 |
|---|---|---|---|
| LOT No. | 04232015 | Lot Specification | distribution |
| Medium | DMEM with 5% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | 1.39-5.80x10^4cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.90x10^6 |
| Viability at cell freezing (%) | 98.3 | Antibiotics Used | free |
| Passage Number | p3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12 D13S317:11,12 D7S820:8,11 D16S539:13 VWA:16,18 TH01:7 AM:X TPOX:8 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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