JCRB0189 KYSE50

Cell information

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Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0189 Cell Name   KYSE50
Profile   Stop providing for the issue of quality control. (Please contact us for more information) Poorly differentiated human squamous cell carcinoma from esophageal cancer. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   58
Identity   available Tissue for Primary Cancer   esophagus
Case history   esophageal cancer, lower intrathoracic esophagus, poorly differentiated squamous cell carcinoma. Metastasis   No
Tissue Metastasized    Genetics   Mode of chromosome number = 105-120. DNA fingerprint indicated the cell line was unique.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cell line was established from inplanted tumor of nude mice (only initial passage).
Classify    Established by   Shimada,Y. et.al.
Registered by   Shimada,Y. Regulation for Distribution   
Comment    Year   2001
Medium   Dulbecco's modified Eagle's medium with 2% fetal calf serum. Methods for Passages   0.25% trypsin and 0.02% EDTA
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   esophagus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1360
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1298222
Reference
Pubmed id:9033652Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines.
Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K
Int J Cancer. 1997 Feb 7;70(4):437-42
Pubmed id:8575860Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors.
Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K
Int J Cancer. 1996 Jan 26;65(3):372-6
Pubmed id:1728357Characterization of 21 newly established esophageal cancer cell lines.
Shimada Y,Imamura M,Wagata T,Yamaguchi N,Tobe T
Cancer. 1992 Jan 15;69(2):277-84

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0189 Cell Name   KYSE50
LOT No.   02182005 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 2% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1.5 - 2.0 x 10^5 cells/ml Methods for Passages   200 units/ml trypsin and 0.01% EDTA
Doubling Time   ca. 2-3 days Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   87.4 Antibiotics Used   free
Passage Number   P6 at bank PDL   
Sterility: MYCOPLASMA   + Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0189 Cell Name   KYSE50
LOT No.   02242015 Lot Specification   distribution
Medium   RPMI1640(GIBCO) and Ham's F12 medium(SiGMA)(1/1) with 2% heat inactivated fetal bovine serum(SIGMA12J396). Temperature   37 C
Cell Density at Seeding   1.80-5.22x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 1mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.32x10^6
Viability at cell freezing (%)   85.7 Antibiotics Used   
Passage Number   p10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:11
D7S820:10
D16S539:10,12
VWA:17,19
TH01:8
AM:X
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   Acholeplasma has eliminated with Plasmocin(Final concentration 25 ug/ml). Expected viability (defrost ampoule) 88.0%.
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