JCRB0186 OV3121ras4

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0186 Cell Name   OV3121ras4
Profile   Mouse ovarian granulosa cell tumor line with v-Ha-ras gene. Other Name   
Animal   mouse Strain   B6C3F1
Genus   Mus Species   musculus
Sex   F Age   6/8-week-old
Identity    Tissue for Primary Cancer   ovary
Case history   Ovarian granulosa cell tumor developed by 60Co gamma-irradiation. Metastasis   
Tissue Metastasized    Genetics   v-Ha-ras gene transfected. pBW1160 was introduced by electropolation method.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Ovarian granulosa cell line with v-Ha-ras gene. Doubling time = 13 hrs.
Classify   transformed Established by   Yanagihara,K.
Registered by   Yanagihara,K. Regulation for Distribution   
Comment    Year   2001
Medium   Dulbecco's modified Eagle's medium with 2mM L-glutamine and 8% heat-inactivated fetal calf serum. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage    Race   
CO2 Conc.    Tissue Sampling   ovary
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_6548
Reference
Pubmed id:7775256A radiation-induced murine ovarian granulosa cell tumor line: introduction of v-ras gene potentiates a high metastatic ability.
Yanagihara K,Nii M,Tsumuraya M,Numoto M,Seito T,Seyama T
Jpn J Cancer Res. 1995 Apr;86(4):347-56

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0186 Cell Name   
LOT No.   10072004 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   7 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   22 hrs Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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