JCRB0178.0 KP-3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0178.0 Cell Name   KP-3
Profile   A metastasis of adenosquamous carcinoma of the human pancreatic duct. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   75
Identity   available Tissue for Primary Cancer   pancreas
Case history   pancreatic tumor Metastasis   Yes
Tissue Metastasized   liver Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   A metastasis of adenosquamous carcinoma of the pancreatic duct.
Classify   tumor Established by   Kono,A. et. al.
Registered by   Iguchi,H. Regulation for Distribution   
Comment   Unique bat the pattern was similar to the KP-3L which is reported to be derived from the KP-3. Year   2001
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells are harvested after treatment with 0.02% EDTA and 0.1% trypsin. Subculture when cells reach to subconfluent.
Cell Number on Passage   1-2 x 10^5 cells/T25 frask Race   Japanese
CO2 Conc.   5 % Tissue Sampling   pancreas
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3005
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1298219
Reference
Pubmed id:9735394Alteration of the CDKN2A gene in pancreatic cancers: Is it a late event in the progression of pancreatic cancer?
Sugimoto Y,Morita R,Hikiji K,Imura G,Ogata Y,Yasuda D,Kono A,Iguchi H
Int J Oncol. 1998 Oct;13(4):669-76
Pubmed id:21607521Alterations of the p53 tumor-suppressor gene and ki-ras oncogene in human pancreatic cancer-derived cell-lines with different metastatic potential.
Iguchi H,Morita R,Yasuda D,Takayanagi R,Ikeda Y,Takada Y,Shimazoe T,Nawata H,Kono A
Oncol Rep. 1994 Nov;1(6):1223-7
Pubmed id:2172194Establishment and characterization of human pancreatic cancer cell lines in tissue culture and in nude mice.
Ikeda Y,Ezaki M,Hayashi I,Yasuda D,Nakayama K,Kono A
Jpn J Cancer Res. 1990 Oct;81(10):987-93

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0178.0 Cell Name   KP-3
LOT No.   12012006 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4518333S) Temperature   37 C
Cell Density at Seeding   4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   97.1 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0178.0 Cell Name   KP-3
LOT No.   09272001 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum(Intergen RB52405). Temperature   37 C
Cell Density at Seeding   2.5 x 10^4 Methods for Passages   Cells harvested after treatment with 0.1% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   95.6 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examine. Human not a HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:9,11
D7S820:10,12
D16S539:9,13
VWA:17,18
TH01:6,10
AM:X
TPOX:9,11
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0178.0 Cell Name   KP-3
LOT No.   07252014 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   4.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   81.0 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:9,11
D7S820:10,12
D16S539:9,13
VWA:18
TH01:6,9.3
AM:X
TPOX:9,11
CSF1PO:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   81.0 Additional information   
Cell No.   JCRB0178.0 Cell Name   KP-3
LOT No.   10112019 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   4 - 8 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.1% trypsin.
Doubling Time   approx. 38 hrs Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   99.3 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
Cell No.   JCRB0178.0 Cell Name   KP-3
LOT No.   01222024 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; NICHIREI Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   0.7 - 2.1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time   approx. 29 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:9,11
D7S820:10,12
D16S539:9,13
VWA:18
TH01:6,9.3
AM:X
TPOX:9,11
CSF1PO:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   90.0 Additional information   
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