JCRB0177.0 KP-1N

Cell information

Important Notice(s)

JCRB0177.0 KP-1N, JCRB0177.1 KP-1NL細胞の由来の問題について

Problem of identity in JCRB0177.0 KP-1N and JCRB0177.1 KP-1NL.

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0177.0 Cell Name   KP-1N
Profile   Stop providing for the issue of quality control. (Please contact us for more information) Human pancreatic cancer cell line established in nude mice. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   69
Identity   available Tissue for Primary Cancer   pancreas
Case history   Pancreatic tumor Metastasis   Yes
Tissue Metastasized   liver metastasis Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Human pancreatic cancer cell line established in nude mice.
Classify   tumor Established by   Kono,A. et. al.
Registered by   Iguchi,H. Regulation for Distribution   
Comment   The STR-PCR pattern was identical with the KP-1NL(JCRB0177.0). Year   2001
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   Cells are harvested after treatment with 0.02% EDTA and 0.1% trypsin. Subculture when cells reached to subconfluent.
Cell Number on Passage   1-2 x 10^5 cells/T25 frask Race   Japanese
CO2 Conc.    Tissue Sampling   pancreatic tumor
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3002
Reference
Pubmed id:9735394Alteration of the CDKN2A gene in pancreatic cancers: Is it a late event in the progression of pancreatic cancer?
Sugimoto Y,Morita R,Hikiji K,Imura G,Ogata Y,Yasuda D,Kono A,Iguchi H
Int J Oncol. 1998 Oct;13(4):669-76
Pubmed id:21607521Alterations of the p53 tumor-suppressor gene and ki-ras oncogene in human pancreatic cancer-derived cell-lines with different metastatic potential.
Iguchi H,Morita R,Yasuda D,Takayanagi R,Ikeda Y,Takada Y,Shimazoe T,Nawata H,Kono A
Oncol Rep. 1994 Nov;1(6):1223-7
Pubmed id:2172194Establishment and characterization of human pancreatic cancer cell lines in tissue culture and in nude mice.
Ikeda Y,Ezaki M,Hayashi I,Yasuda D,Nakayama K,Kono A
Jpn J Cancer Res. 1990 Oct;81(10):987-93

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0177.0 Cell Name   
LOT No.   05222006 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   4 - 7 x 10^4 cells/ml Methods for Passages   0.1% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   98.7 Antibiotics Used   free
Passage Number   Unknown (18 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0177.0 Cell Name   KP-1N
LOT No.   08122002 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (Mitsubishi PVF001). Temperature   37 C
Cell Density at Seeding   2.1 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.1% trypsin. Medium change twice a week. Subculture once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   98.2 Antibiotics Used   free
Passage Number   P16* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(type B), NP and LDH examined. Human,not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:11
D7S820:8,10
D16S539:11
VWA:15
TH01:7,8
AM:X
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0177.0 Cell Name   KP-1N
LOT No.   08242001 Lot Specification   distribution
Medium   RPMI1640 medium with 10 % fetal calf serum(Intergen RB52405) and 5ug/ml MC210. Temperature   37 C
Cell Density at Seeding   2.0 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.1% trypsin. Subculture once or twice a week and medium change in between.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   99.3 Antibiotics Used   MC210 (5 ug/ml). Only for this cell culture.
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   + Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human, not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO, but no MC210 included. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
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