JCRB0170 Lu-135

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0170 Cell Name   Lu-135
Profile   Human small cell lung carcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   69
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   lung small cell carcinoma, variant type (NCI indicator). Metastasis   
Tissue Metastasized    Genetics   Neuron specific enolase(NSE), creatin kinase isoenzyme(CKBB) were indicated to be high activity but aromatic L-amino acid decarboxylase(AADC) had low activity.
Life Span   infinite Crisis PDL   
Morphology   round and aggregated Character   Hard to observe dense core granules (neurosecretory granules) under electron microscopic observation. Doubling time was 45 hours reported originally.
Classify   tumor Established by   Shimosato,Y.
Registered by   Terasaki,T. Regulation for Distribution   
Comment    Year   2001
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   When cells are harvested, remove culture medium then add 1ml of fresh medium to the cells. Cells are then dispersed by pipetting and 1 ml of the cell suspension should be added to the 9 ml of fresh medium.
Cell Number on Passage   split ratio = 1/10 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1389
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 713899
Reference
Pubmed id:2856251Altered expression of the retinoblastoma (RB) gene in small-cell carcinoma of the lung.
Yokota J,Akiyama T,Fung YK,Benedict WF,Namba Y,Hanaoka M,Wada M,Terasaki T,Shimosato Y,Sugimura T
Oncogene. 1988 Oct;3(4):471-5
Pubmed id:2838790Heterogeneity of lung cancer cells with respect to the amplification and rearrangement of myc family oncogenes.
Yokota J,Wada M,Yoshida T,Noguchi M,Terasaki T,Shimosato Y,Sugimura T,Terada M
Oncogene. 1988 Jun;2(6):607-11
Pubmed id:3022030Changes in cell characteristics due to culture conditions in cell lines from human small cell lung cancer.
Terasaki T,Shimosato Y,Nakajima T,Tsumuraya M,Morinaga S,Hirohashi S,Yamaguchi K,Kato K,Ichinose H,Nagatsu T
Jpn J Clin Oncol. 1986 Sep;16(3):203-12

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0170 Cell Name   
LOT No.   06032003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   1-1.5 x 10^5 cells/ml Methods for Passages   Pipetting
Doubling Time   D.T. = ca. 2 days Cell Number in Vial (cells/1ml)   5.1 x 10^5
Viability at cell freezing (%)   59 Antibiotics Used   free
Passage Number   P263 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD and LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:7
D7S820:11
D16S539:11
VWA:16
TH01:9
AM:X,Y
TPOX:11
CSF1PO:10
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0170 Cell Name   Lu-135
LOT No.   09062017 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.2 - 3.1 x 10^5 Methods for Passages   Cells were dispersed by pipetting. Part of cells will loosely attach to the substratum.
Doubling Time   approx. 44 hrs. Cell Number in Vial (cells/1ml)   1.0 x 10^6 (absolute No. of viable cells)
Viability at cell freezing (%)   39.0 Antibiotics Used   free
Passage Number   P262 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   39.0 Additional information   
Cell No.   JCRB0170 Cell Name   Lu-135
LOT No.   03112024 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   1.8 - 6.7 x 10^5 cells/mL Methods for Passages   Cells were dispersed by pipetting. Part of cells will loosely attach to the substratum.
Doubling Time   approx. 47 hrs. Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   76 Antibiotics Used   free
Passage Number   P262 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   70 Additional information   Cells will grow with forming aggregates.
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