JCRB0169 Lu-134-B

Cell information

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Culture medium for JCRB0169 Lu-134-B

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0169 Cell Name   Lu-134-B
Profile   Small cell lung carcinoma cell line established through heterotransplanted tumor. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   80
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   small cell carcinoma arising in the periphery of right lower lobe of lung. Metastasis   
Tissue Metastasized    Genetics   Activities of gamma-enolase and aromatic L-amino acid decarboxylase are high significantly. Hypertetraploid chromosome pattern. M=85, Td=85 hrs
Life Span   infinite Crisis PDL   
Morphology   Cluster formation. Character   Derived from small cell lung carcinoma, classic type. Cells are prepared from heterotransplantation of the tumor into athymic BALB/c mice. Dense cored granule (neurosecretory granule) observed unde EM.
Classify   tumor Established by   Terasaki,T. and Shimosato,Y.
Registered by   Terasaki,T. Regulation for Distribution   
Comment    Year   2001
Medium   Eagle's minimal essential medium supplemented with glutamine and 10% heat-inactivated fetal calf serum. Methods for Passages   Same volume of a fresh medium as the cell culture was added to the cell suspension and pipetting twice or three times. Take a portion of the diluted cell suspension is applied to further cell culture.
Cell Number on Passage   split ratio = 1/2. Race   Japanse
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1388
Reference
Pubmed id:2168867Selection of radioresistant cells by vitamin A deficiency in a small cell lung cancer cell line.
Terasaki T,Shimosato Y,Wada M,Yokota J,Terada M
Jpn J Cancer Res. 1990 Aug;81(8):780-5
Pubmed id:2438038Reversible squamous cell characteristics induced by vitamin A deficiency in a small cell lung cancer cell line.
Terasaki T,Shimosato Y,Nakajima T,Tsumuraya M,Ichinose H,Nagatsu T,Kato K
Cancer Res. 1987 Jul 1;47(13):3533-7
Pubmed id:6098504Interconversion of biological characteristics of small cell lung cancer depending on culture conditions.
Terasaki T,Kameya T,Nakajima T,Tsumuraya M,Shimosato Y,Kato K,Ichinose H,Nagatsu T,Hasegawa T
Gan. 1984 Dec;75(12):1089-99

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0169 Cell Name   
LOT No.   06022003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   uncountable due to aggregates. Split ratio 1:6-1:3 Methods for Passages   Dilution with pipetting large cell aggregates to small fragments.
Doubling Time    Cell Number in Vial (cells/1ml)   5 - 10 x 10^6
Viability at cell freezing (%)   ca. 70 Antibiotics Used   free
Passage Number   > P31 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD and LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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