JCRB0167 RCN-9
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0167 | Cell Name | RCN-9 |
|---|---|---|---|
| Profile | DMH induced colonic cancer, (F344/DuCrj rat) | Other Name | RCN-9 |
| Animal | rat | Strain | |
| Genus | Rattus | Species | norvegicus |
| Sex | M | Age | 20-week |
| Identity | Tissue for Primary Cancer | colon | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | DMH induced colonic cancer. Male F344/DuCrj rat. | |
| Life Span | Crisis PDL | ||
| Morphology | Character | DMH induced colonic cancer. Male F344/DuCrj rat. | |
| Classify | Established by | Inoue,Y. & Kashima,Y | |
| Registered by | Inoue, Y. et al. | Regulation for Distribution | |
| Comment | Low differentiated adenocarcinoma. | Year | 2001 |
| Medium | RPMI1640 medium with 10% fetal calf serum. | Methods for Passages | Cells are treated with 0.02 % EDTA and 0.25 % trypsin. |
| Cell Number on Passage | 2x10^5 cells/60mm dish | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | colon |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:1900274 | A new rat colon cancer cell line metastasizes spontaneously: biologic characteristics and chemotherapeutic response. Inoue Y,Kashima Y,Aizawa K,Hatakeyama K Jpn J Cancer Res. 1991 Jan;82(1):90-7 |
| Research results by users. Click！ | |
|---|---|
| Pubmed id:9655087 |
Involvement of liver-associated immunity in hepatic metastasis formation. Okuno K,Hirai N,Lee YS,Kawai I,Shigeoka H,Yasutomi M J Surg Res. 1998 Mar;75(2):148-52 |
| Images |
|---|
     |
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0167 | Cell Name | |
|---|---|---|---|
| LOT No. | 06202002 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (lot; BioWhittaker 0S069F) | Temperature | 37 C |
| Cell Density at Seeding | 1.0 x 10^5 cells/ml or lower. | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | 4.1 x 10^6 | |
| Viability at cell freezing (%) | 95.6 | Antibiotics Used | free |
| Passage Number | P129 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as rat by NP, G6PD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS-DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |