JCRB0163 HL60(S)

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0163 Cell Name   HL60(S)
Profile   Promyeloblastic cell line differentiated to neutrophils or macropahges by tumor promoters, vitamne D3 or cytekines. This HL60(S) has been independently obtained by Dr. Ishida,S. and Dr. Shudo,K. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   36
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute promyelotic leukemia Metastasis   
Tissue Metastasized    Genetics   APL
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   DMSO, butyl, HX., TPA, active vitamine D3, and Retinoic Acid induce differentiation efficiently.
Classify   tumor Established by   Collins,S.J. et al.
Registered by   Shudo,K. Regulation for Distribution   free
Comment   STR-PCR results indicated the HL60(S) is the same with HL60(JCRB0085) Year   2000
Medium   RPMI1640 medium with 5% fetal bovine serum (heat inactivation may be done). Methods for Passages   Simple dilution. Subculture every 3 days.
Cell Number on Passage   1 x 10^5 cells/ml Race   Cocasian
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2944
Reference
Pubmed id:7944409Induction of differentiation and covalent binding to proteins by the synthetic retinoids Ch55 and Am80.
Takahashi N,Breitman TR
Arch Biochem Biophys. 1994 Oct;314(1):82-9
Pubmed id:3184125Retinobenzoic acids. 1. Structure-activity relationships of aromatic amides with retinoidal activity.
Kagechika H,Kawachi E,Hashimoto Y,Himi T,Shudo K
J Med Chem. 1988 Nov;31(11):2182-92
Pubmed id:6192693Fundamentals of chemotherapy of myeloid leukemia by induction of leukemia cell differentiation.
Hozumi M
Adv Cancer Res. 1983;38():121-69
Pubmed id:288488Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia.
Gallagher R,Collins S,Trujillo J,McCredie K,Ahearn M,Tsai S,Metzgar R,Aulakh G,Ting R,Ruscetti F,Gallo R
Blood. 1979 Sep;54(3):713-33
Pubmed id:271272Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture.
Collins SJ,Gallo RC,Gallagher RE
Nature. 1977 Nov 24;270(5635):347-9

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0163 Cell Name   
LOT No.   07292004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum. At log growth phase, serum contents can be decreased to 5%. (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   7-10 x 10^4 cells/ml Methods for Passages   dilution
Doubling Time   21 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   P7 at bank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0163 Cell Name   HL60(S)
LOT No.   04192001 Lot Specification   distribution
Medium   RPMI1640 medium with 10 or 20% heat inactivated fetal bovine serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   2.0 x 10^5 cells/ml Methods for Passages   Simple dilution. split ratio=1/4. Subculture every 3-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   94.1 Antibiotics Used   free
Passage Number   P3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5 % DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0163 Cell Name   HL60(S)
LOT No.   04112017 Lot Specification   distribution
Medium   RPMI 1640 medium with 10 - 20% fetal bovine serum. At log growth phase, serum contents can be decreased to 5%. (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 18 hrs. Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   88.1 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   88.1 Additional information   
Cell No.   JCRB0163 Cell Name   HL60(S)
LOT No.   10212022 Lot Specification   distribution
Medium   RPMI1640(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.76-3.0x10^4 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.68x10^6
Viability at cell freezing (%)   84 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:8,11
D7S820:11,12
D16S539:11
VWA:16
TH01:7,8
AM:X
TPOX:8,11
CSF1PO:13,14
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
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