IFO50516 TMD5

Cell information

Important Notice(s)

On the descripancy of CD antigen expression in TMD5 cells.

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50516 Cell Name   TMD5
Profile   leukemia, acute B lymphoblastic, double Philadelphia chromosomes Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   19
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute lymphoblastic leukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   ovoid Character   leukemia, acute B lymphoblastic, double Philadelphia chromosomes
Classify   tumor Established by   Tohda, S.
Registered by   Tohda,S. Regulation for Distribution   
Comment   Mycoplasmal contamination was eliminated with antibiotic treatment at the IFO. Year   1999
Medium   alpha minimal essential medium (MEM alpha) with 10% fetal bovine serum Methods for Passages   dilution, split ratio 1:6 at 7 days interval
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3218
Reference
Pubmed id:10071078Establishment of a double Philadelphia chromosome-positive acute lymphoblastic leukemia-derived cell line, TMD5: effects of cytokines and differentiation inducers on growth of the cells.
Tohda S,Sakashita C,Fukuda T,Murakami N,Nara N
Leuk Res. 1999 Mar;23(3):255-61

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50516 Cell Name   
LOT No.   02242011 Lot Specification   distribution
Medium   alpha-MEM with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   3.3 - 5.9 x 10^5 cells/mL Methods for Passages   Simple dilution
Doubling Time   approx. 4 days Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   90.3 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - alpha-MEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50516 Cell Name   TMD5
LOT No.   1153 Lot Specification   distribution
Medium   alpha-MEM, 90%; FBS, 10% Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown(6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   MD, G6PD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.)-culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50516 Cell Name   TMD5
LOT No.   06212013 Lot Specification   distribution
Medium   MEM alpha with 10% heat inactivated bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.2-1.0x10^6 cells/ml Methods for Passages   Dilution (split ratio=1/2)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.97x10^6
Viability at cell freezing (%)   94.3 Antibiotics Used   
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,13
D13S317:8
D7S820:10,11
D16S539:10,12
VWA:17,18
TH01:9
AM:X,Y
TPOX:8
CSF1PO:10,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
CLOSE